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Available ordering format
Lyophilized
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Immunogen
peptide of Arabidopsis thaliana AGO5 UniProt: Q9SJK3, TAIR:AT2G27880
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum in PBS pH 7.4.
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunoprecipitation (IP)
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Connected products
AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibodyAS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated) AS09 527B | Anti-AGO1 | argonaute 1 (40 µg, Biotin conjugated) AS09 527-HRP | Anti-AGO1 | argonaute 1 (40 µg, HRP-conjugated) AS14 2776 | Anti-AGO1 | argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P | AGO1 | argonaute 1 | Blocking peptideAS13 3682 | Anti-AGO2 | argonaute 2, rabbit antibodyAS09 617 | Anti-AGO4 argonaute 4, rabbit antibodyAS10 672 | Anti-AGO6 | argonaute 6, rabbit antibodyAS10 673 | Anti-AGO9 | argonaute 9, rabbit antibodyAS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibodycollection of antibodies to micro RNAPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 1500 (WB), 5 ug per gram floral tissue (IP)
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Molecular weight expected аpparent
111 | 111 kDa
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Arabidopsis thaliana
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No reactivity
Zea mays
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Supplementary information
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. TCA acetone total protein precipitation method
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References
To be added when available, antibdy released in August 2012.
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Scientific context
AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). Probably involved in antiviral RNA silencing.
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Notes
None
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Protein number
Q9SJK3
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TAIR number
AT2G27880
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