-
Available ordering format
Lyophilized
-
Immunogen
KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5 , TAIR: At2g27040.
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Affinity purified serum in PBS pH 7.4.
-
How to reconstitute
For reconstitution add 50 µl of sterile water.
-
Storage condition
Store at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tubes.
-
Verified applications
western blot (WB), immunoprecipitation (IP), coimmunoprecipitation (coIP), immunocytochemistry (ICC)
-
Connected products
AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibodyAS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated) AS09 527B | Anti-AGO1 | argonaute 1 (40 µg, Biotin conjugated) AS09 527-HRP | Anti-AGO1 | argonaute 1 (40 µg, HRP-conjugated) AS14 2776 | Anti-AGO1 | argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P | AGO1 | argonaute 1 | Blocking peptideAS13 3682 | Anti-AGO2 | argonaute 2, rabbit antibodyAS10 671 | Anti-AGO5 | argonaute 5, rabbit antibodyAS10 672 | Anti-AGO6 | argonaute 6, rabbit antibodyAS10 673 | Anti-AGO9 | argonaute 9, rabbit antibodyAS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibodycollection of antibodies to micro RNASecondary antibodies
-
Recommended dilutions for use
1: 4000 (WB), 5 µg of antibody per 1 gram of a fresh tissue (IP), 1: 100 (ICC)
-
Molecular weight expected аpparent
103 kDa
-
Verified reactivity
Arabidopsis thaliana, Hyacinthus orientalis
-
Possible reactivity
Arabidopsis thaliana
-
No reactivity
Brassica oleracea, Hordeum vulgare, Solanum lycopersicum, Zea mays, Vigna angularis
-
Supplementary information
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Note that the AGO4 antibody reacts with the NEB prestained protein marker.
-
References
Zhai et al. (2015). A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis. Cell. 2015 Oct 8;163(2):445-55. doi: 10.1016/j.cell.2015.09.032. Han et al. (2014). SUVR2 is involved in transcriptional gene silencing by associating with SNF2-related chromatin-remodeling proteins in Arabidopsis. Cell Res. 2014 Nov 25. doi: 10.1038/cr.2014.156. (coimmunoprecipitation)Zhang et al. (2013). DTF1 is a core component of RNA-directed DNA methylation and may assist in the recruitment of Pol IV. PNAS, May 14. Havecker et al. (2010) The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. Plant Cell 22(2): 321-34.
-
Scientific context
AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
-
Notes
Binds endogenous siRNAs, preference for 24nt siRNAs with 5' A.TCA acetone total protein precipitation method
-
Protein number
Q9ZVD5
-
TAIR number
AT2G27040
-
