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Available ordering format
Liquid, conjugated to biotin.
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Immunogen
N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR:At1g48410.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum in PBS pH 7.4.
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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Verified applications
western blot (WB)
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Connected products
AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibodyAS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated) AS09 527-HRP | Anti-AGO1 | argonaute 1 (40 µg, HRP-conjugated) AS14 2776 | Anti-AGO1 | argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P | AGO1 | argonaute 1 | Blocking peptideAS13 3682 | Anti-AGO2 | argonaute 2, rabbit antibodyAS09 617 | Anti-AGO4 argonaute 4, rabbit antibodyAS10 671 | Anti-AGO5 | argonaute 5, rabbit antibodyAS10 672 | Anti-AGO6 | argonaute 6, rabbit antibodyAS10 673 | Anti-AGO9 | argonaute 9, rabbit antibodyAS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibodycollection of antibodies to micro RNAPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 1000 (WB)
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Molecular weight expected аpparent
116.4 | 130 kDa
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera
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No reactivity
Chlamydomonas reinhardtii
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Supplementary information
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
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References
To be added when available, antibody released in November 2015.
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Scientific context
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Biotin.
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Notes
Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.TCA acetone total protein precipitation method
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Protein number
O04379
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TAIR number
AT1G48410
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
