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Available ordering format
Liquid, contains 50% glycerol
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Immunogen
full length ACT11 (Actin-11) from Arabidopsis thaliana, accession number NP_187818.1
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
IgG2b
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Purification
Affinity purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at -20°C.Shelf life of this product is one year from the date of shipment.
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Verified applications
western blot (WB), immunofluorescence (IF)
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Connected products
AS13 2640 | anti-ACT | actin, rabbit antibody AS10 681 | Anti-tubulin beta chain, rabbit antibodyAS10 680 | Anti-tubulin alpha chain, rabbit antibodyAS16 3140 | Actin-2, mouse monoclonal antibodyAS16 3141 | Actin-8, mouse monoclonal antibodyAS16 3139 | Actin-1, monoclonal antibodyPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 2000 with standard ECL (WB), 1: 700 (IF)
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Molecular weight expected аpparent
41.6 | 45 kDa
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Verified reactivity
Arabidopsis thaliana, Fusarium oxysporum f.sp. cubense, Nepeta cataria, Nepeta rtanjensis, Nicotiana tabacum, Oryza sativa, Solanum tuberosum, Zea mays
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Possible reactivity
dicots including: Glycine max, Pisum sativun, Ricinus communis, monocots, trees: Picea abies, Chlamydomonas reinhardtii
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
This antibody is not suitable as a loading control for tissues from various organs of cauliflower including seeds.
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References
Deng et al. (2015). Proteomic analysis of conidia germination in Fusarium oxysporum f. sp. cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling Fusarium wilt of banana. Appl Microbiol Biotechnol. 2015 Sep;99(17):7189-207. doi: 10.1007/s00253-015-6768-x. Epub 2015 Jul 1. Dmitrovic et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64. Weiste and Dröge-Laser (2014). The Arabidopsis transcription factor bZIP11 activates auxin-mediated transcription by recruiting the histone acetylation machinery. Nat Commun. 2014 May 27;5:3883. doi: 10.1038/ncomms4883. Weits et al. (2014). Plant cysteine oxidases control the oxygen-dependent branch of the N-end-rule pathway. Nat Commun. 2014 Mar 6;5:3425. doi: 10.1038/ncomms4425.
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Scientific context
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
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Notes
Antibody has been affinity purified.
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Protein number
Refer to NCBI
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TAIR number
AT3G12110
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