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Available ordering format
Liquid in PBS pH 7.4 and 50 % glycerol
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Immunogen
chemically modified KLH allowing acetylation of all lysines of this carrier protein
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at +4°C
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Verified applications
western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), ELISA
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Connected products
AS15 3018 | anti-Acetylated Lysine, rabbit antibodies AS10 707-25 | anti-Acetylated lysine (25 µl), mouse antibodyAS10 707-100 | anti-Acetylated lysine (100 µl), mouse antibody AS15 3019 | anti-Acetylated Lysine, APL conjugated, rabbit antibodycollection of antibodies to epigeneticsPlant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1:250 (WB), 1:100 (ICC), 1: 100 (IF)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Detects proteins containing acetylated lysine residues in all specias.
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Possible reactivity
other species
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No reactivity
No reaction to non-acetylated proteins.
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Supplementary information
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References
to be added when available, antibody released in October 2015.
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Scientific context
Acetylated Lysine is involved in post-translational modifications of proteins which play a critical role in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation.This antibody is conjugated to FITC.
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Notes
Immunogen affinity purified antibodies are present in PBS with 0.09 % sodium azide and 50 % glycerol.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
This agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
