2, Set of 10 plant anti-Lhca and anti-Lhcb antibodies

  • Catalog number
    AS01 011
  • Price
    Please ask
  • Size
    9 x 200 µl + 1 x 50 µg (Lhcb1 antibody)
  • Available ordering format
    Lyophilized
  • Immunogen
    BSA or KLH-conjugated synthetic peptides derived from conserved regions of plant Lhca1-4 and Lhcb1-6 protein sequences.
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Total IgG in PBS pH 7.4, affinity purified serum (Lhcb1) or serum (Lhcb5)
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    other antibodies to pigment-binding proteinsrecommended secondary antibodyPlant protein extraction bufferSecondary antibodies
  • Recommended dilutions for use
    1:2000 - 1:10 000 with standard ECL (WB)
  • Molecular weight expected аpparent
    20 - 29 kDa
  • Verified reactivity
    Higher plants including A.thaliana, H.vulgare, P.abies, S.oleracea, Drosera capensisThese antibodies have been shown to be reactive in all dicots, monocots, and gymnosperms tested so far; some of them have even been found to be reactive against Lhc-proteins of Chlamydomonas reinhardtii
  • Possible reactivity
    to be determined
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    Contact Gentaur support center at [email protected]
  • References
    Pavlovic et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008Xu et al. (2011). Light-harvesting chlorophyll a/b-binding proteins are required for stomatal response to abscisic acid in Arabidopsis. J. Ex. Bot. Dec 5 (ahead of print).Kang et al. (2010). Evaluation of light-harvesting complex proteins as senescence-related protein markers in detached rice leaves. Photosynthetica 47, 4:638-640.
  • Scientific context
    In plants the outer light-harvesting antenna of the photosystems (PSI and PSII) is composed of pigment-binding proteins belonging to the Lhc family. Of the 10 main conserved subtypes Lhca1-4 are exclusively associated with PSI while Lhcb3-6 are only found with PSII. Lhcb1 and Lhcb2 are found in the mobile trimers of LHCII associating with both photosystems in variable ratios (state1-state2 transitions). All Lhc polypeptides bind an individual set of chlorophyll a, chlorophyll b, and xanthophyll molecules in a structural and dynamic mutual arrangement which ensures high efficiency of energy transfer processes involved in light-harvesting and photoprotection.
  • Notes
    This set contains the following antibodies:Product number:Product name: AS01 005AS01 006AS01 007AS01 008AS01 004AS01 003AS01 002AS04 045AS01 009AS01 010Anti-Lhca1Anti-Lhca2Anti-Lhca3Anti-Lhca4Anti-LhcbAnti-Lhcb2Anti-Lhcb3Anti-Lhcb4Anti-Lhcb5Anti-Lhcb6
  • Protein number
    Q01667 , Q9SYW8 , Q8LCQ4 , Q9SY97 , P27521 , Q8VZ87 , P04777 , P04778 , Q9C5R6 , Q39141 , Q9SHR7 , Q9S7J7 , Q9XF87 , Q9XF86 , Q9S7M0 , Q9LMQ2
  • TAIR number
    AT1G29910, AT1G29920, AT1G29930, AT2G34420, AT2G34430, AT5G54270,AT4G10340,AT1G15820, AT3G61470,AT3G54890, AT1G61520, AT3G47470
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Plants RNA, DNA , cDNA and plant tissues are used to study plant genes.
  • Gene target
    Set   Lhca   Lhcb   antibodies  
  • Short name
    2, Set of 10 anti-Lhca anti-Lhcb antibodies
  • Technique
    anti-, anti, antibody to, antibodies
  • Species
    Plant, Plants
  • Alternative name
    2, Set on 10 vegetal Antibody toLhca and Antibody toLhcb antibodies
  • Alternative technique
    antibodies
  • Tissue
    set
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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