2, Rubisco quantitation kit

• Catalog number
  AS09 409
• Price
  Please ask
• Size
  1 x 50 µl of AS03 037, RbcL | Rubisco large subunit, form I and form II (amount enough for 50-100 Western blots)1 x 100 µl of AS01 017S, Rubisco protein standard (0.15 pmoles / µl, amount enough for generation of standard curve in 85assays (standard curve: 0.0625 pmoles, 0.125 pmoles, 0.25 pmoles)2 x 2 ml AS08 300, Protein extraction buffer (amount enough for 48 isolations of plant material, using 500 µl 1x PEB for 100 mg fresh weight) or 120 isolations of algal material (using 200 µl 1x PEB for cell amounts corresponding to 4-10 µg total chlorophyll)1 x 50 µl of AS09 602, Goat anti-rabbit IgG (H&L), HRP conjugated(amount enough for 50-100 Western blots)

• Available ordering format
  Inquire
• Immunogen
  KLH-conjugated synthetic peptide was used to elicit anti-RbcL antibody. No baculovirus was used in production of this product.
• Raised in
  Rabbit. Secondary antibody: Goat.
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  RbcL antibody and protein standard:Please stoore at -20°C (6 month) or -80°C for long term storage(years). Please, avoid freezing and thawing of reconstituted antibodies, make aliquots instead.PEB extraction buffer:stable at RT for at least 1 month. For short term storage please stoore (1 month) at +4°C and for long term storage (1 year) at -20°C.
• Verified applications
  Western blot (WB)
• Connected products
  AS03 037 | anti-RbcL | Rubisco large subunit, form I and form II (50 µl)AS03 037A | anti-RbcL | Rubisco large subunit, form I and form II (50 µg affinity purified)AS03 037-HRP| anti-RbcL | Rubisco large subunit, form I and form II (40 µg, HRP-conjugated) AS15 2994 | Rubisco ELISA quantitation kit AS15 2955 | anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibodyAS15 2955S | RbcL II | Rubisco form II positive control/quantitation standardAS01 017 | anti-RbcL | Rubisco large subunit, form I, chicken antibodyAS01 017S | Rubisco protein standard for quantitative western blot or positive controlAS03 037PRE | Rubisco large subunit, pre-immune serum AS07 218 | anti-Rubisco | 557 kDa hexadecamer, rabbit antibody to a whole protein AS07 259 | anti-RbcS | Rubisco small subunit (SSU), rabbit antibodyAS07 222 | anti-RbcS | Rubisco small subunit (SSU) from pea, rabbit antibody matching secondary antibody
• Recommended dilutions for use
  1:5000-1:10000 with standard ECL (WB)
• Molecular weight expected аpparent
  Rubisco protein standard: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
• Verified reactivity
  Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Emiliana huxleyi, Euglena gracilis, Gonyaulax polyedra, Heterosigma akashiwo, Micromonas pusila, Porphyra sp. Spinacia oleracea, Synechococcus PCC 7942, Thalassiosira pseudonana
• Possible reactivity
  di and monocots, conifers, mosses, liverworts, welwitschia, green algae, red alge, brown algae, cryptomonad, cyanobacteria including prochlorophytes, gamma-proeobacteria, beta-proteobacteria, alpha proteobacteria
• No reactivity
  to be determined
• Supplementary information
  Quantitative western blot: detailed method description, video tutorialDiscussion over some critical aspects of quantitative western blot: Heidebrecht et al. (2009). Improved semiquantitative Western blot technique with increased quantification range. J. Immunological Methods. 35:40-48.
• References
  Mota et al. (2015). Effects of heavy metals on Cyanothece sp. CCY 0110 growth, extracellular polymeric substances (EPS) production, ultrastructure and protein profiles. J Proteomics. 2015 Apr 29;120:75-94. doi: 10.1016/j.jprot.2015.03.004. Thamatrakoln et al. (2013). Death-specific protein in a marine diatom regulates photosynthetic responses to iron and light availability. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20123-8. doi: 10.1073/pnas.1304727110. Supplemental material describes western blot quantification method.
• Scientific context
  The Rubisco quantitation kit is suitable for quantitation of Rubisco in plant and algal samples using quantitative immunoblotting. It contains anti-RbcL antibody, Rubisco protein standard and the Protein Extraction Buffer for quantitative protein extraction. This kit is a good alternative to quantification of Rubisco by ELISA as it allows separation of Rubisco from a total complex cell protein mixture, which can be troublesome in case of ELISA method.
• Notes
  Recommended secondary antibody: AS09 602, Goat anti-rabbit IgG (H&L), HRP conjugated
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink

• Gene target
  Rubisco   quantitation   kit  
• Short name
  2, Rubisco quantitation kit
• Alternative name
  2, Rubisco quantitation reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products