anti-FITC F(ab)2 Fragment Antibody, 1 mg

  • Catalog number
    AP09428BT-N
  • Price
    Please ask
  • Size
    1 mg
  • Vial description
    Fluorescein Isothiocyanate
  • Category
    primary antibody
  • Raised in
    Goat
  • Clone
    1 vial
  • Form
    Biotin
  • Reactivity
    not determined
  • Purity
    purified
  • Molecular weight
    in kDa
  • Source
    mammalian
  • Activity
    to be determined
  • Range
    broad
  • Protein number
    see ncbi
  • Application
    ELISA, Western Blot
  • Storage
    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by acr they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This acr Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. acr FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • French translation
    anticorps
  • Gene target
    -
  • Short name
    anti-FITC F(ab)2 Antibody, 1 mg
  • Technique
    Antibody, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein
  • Isotype
    IgG, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
  • Label
    FITC
  • Species
    human
  • Alternative name
    Antibody toFITC binding fragment 2 Fragment (antibody to-), 1 mg
  • Alternative technique
    antibodies, fluorescine
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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