anti-FITC F(ab)2 Fragment Antibody, 1 mg
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Catalog number
AP09428BT-N
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Price
Please ask
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Size
1 mg
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Vial description
Fluorescein Isothiocyanate
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Category
primary antibody
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Raised in
Goat
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Clone
1 vial
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Form
Biotin
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Reactivity
not determined
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Purity
purified
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Molecular weight
in kDa
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Source
mammalian
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Activity
to be determined
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Range
broad
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Protein number
see ncbi
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Application
ELISA, Western Blot
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Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by acr they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This acr Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. acr FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
anti-FITC F(ab)2 Antibody, 1 mg
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Technique
Antibody, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein
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Isotype
IgG, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
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Label
FITC
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Species
human
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Alternative name
Antibody toFITC binding fragment 2 Fragment (antibody to-), 1 mg
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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