anti-FITC Antibody, 0,2 mg
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Catalog number
AM20262HR-N
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Price
Please ask
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Size
0,2 mg
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Vial description
Fluorescein Isothiocyanate
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Category
primary antibody
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Clonality
monoclonal
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Raised in
Mouse
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Clone
NI 239
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Form
HRP
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Reactivity
not determined
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Purity
purified
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Molecular weight
in kDa
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Source
mammalian
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Activity
to be determined
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Range
broad
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Protein number
see ncbi
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Application
ELISA, Immunocytochemistry/Immunofluorescence, Western Blot
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Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by acr they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This acr Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. acr FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
anti-FITC Antibody, 2 mg
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Technique
Antibody, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein
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Isotype
IgG1
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Label
FITC
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Species
human
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Alternative name
Antibody toFITC (antibody to-), 0,2 mg
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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