V5-TAG, Mouse Monoclonal antibody-; Clone: SV5-Pk4, IF,flow, FITC conj.
-
Catalog numberYSRTMCA2894F
-
PricePlease ask
-
Size0.1 mg
-
-
Alternative name 1V5-TAG, Mab anti-; Clone: SV5-Pk4, IF,flow, FITC conj.
-
Alternative name 2V5-TAG, Mab antibody-; Clone: SV5-Pk4, IF,flow, FITC conj.
-
Alternative name 3V5-TAG, Mab antibody-; Clone: SV5-Pk4, IF,flow, FITC conj.
-
Alternative name 4anti-V5-TAG Clone: SV5-Pk4 flow
-
Alternative name 5V5-TAG Clone: SV5-Pk4 flow
-
ClonalityMonoclonal Antibody
-
DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
-
Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
-
AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
-
PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
-
ConjugationAnti-FITC Antibody
-
TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Latin nameMus musculus
-
French translationanticorps
-
Gene target
-
Gene symbolLINC01194, CNTN2, TRL-TAG3-1, TRL-TAG1-1, TRL-TAG2-1, TRL-TAG4-1
-
Short nameV5-TAG, Mouse Monoclonal antibody-; Clone: SV5-Pk4, IF, , FITC conj.
-
TechniqueMouse monoclonal, Antibody, Mouse, FITC, Flow, IF, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, Tags
-
Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
-
Labeltag
-
SpeciesMouse, Mouses
-
Alternative nameV5-detection labelled, Mouse monoclonal (antibody to-)-; clonality: SV5-Pk4, IF,flow, fluorecein conj.
-
Alternative techniqueantibodies, murine, fluorescine
-
Gene info
-
Identity
-
Gene
-
Long gene namelong intergenic non-protein coding RNA 1194
-
Synonyms gene
-
Synonyms gene name
- cancer/testis antigen 49
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year2012-11-30
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Long intergenic non-protein coding RNAs
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namecontactin 2
-
Synonyms gene
-
Synonyms gene name
- contactin 2 (axonal)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-05-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Contactins
- I-set domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-Leu (anticodon TAG) 3-1
-
Synonyms gene
-
Synonyms gene name
- transfer RNA leucine 7 (anticodon UAG)
- transfer RNA-Leu (TAG) 3-1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2008-08-29
-
Entrez gene record
-
Classification
- Cytoplasmic transfer RNAs
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-Leu (anticodon TAG) 1-1
-
Synonyms gene
-
Synonyms gene name
- tRNA leucine 2
- tRNA leucine 2 (anticodon UAG)
- transfer RNA leucine 2 (anticodon UAG)
- transfer RNA-Leu (TAG) 1-1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1989-06-30
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Cytoplasmic transfer RNAs
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-Leu (anticodon TAG) 2-1
-
Synonyms gene
-
Synonyms gene name
- transfer RNA leucine 15 (anticodon UAG)
- transfer RNA-Leu (TAG) 2-1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2008-08-29
-
Entrez gene record
-
Classification
- Cytoplasmic transfer RNAs
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-Leu (anticodon TAG) 4-1
-
Synonyms gene
-
Synonyms gene name
- transfer RNA leucine 40 (anticodon UAG) pseudogene
- transfer RNA-Leu (TAG) 4-1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2008-08-29
-
Entrez gene record
-
RefSeq identity
-
Classification
- Cytoplasmic transfer RNA pseudogenes
MeSH Data
-
Name
-
ConceptScope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
-
Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data