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Alternative name 1
Microphthalmia Transcription Factor (MiTF), 52-56kD, Clone: C5, Mab anti-Human, Horse, Dog, Mouse, Rat; paraffin, IH/WB
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Alternative name 2
Microphthalmia Transcription Factor (MiTF), 52-56kD, Clone: C5, Mab antibody-Human, Horse, Dog, Mouse, Rat; paraffin, IH/WB
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Alternative name 3
Microphthalmia Transcription Factor (MiTF), 52-56kD, Clone: C5, Mab antibody-Homo sapiens, Horse, Canine, Mus musculus, Rattus norvegicus; paraffin, IH/WB
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Alternative name 4
anti-Microphthalmia Transcription Factor MiTF 52-56kD Clone: C5 Horse
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Alternative name 5
Microphthalmia Transcription Factor MiTF 52-56kD Clone: C5 Horse
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Canine or Canis Lupus is mostly Beagle used for drug research. accurate-monoclonals produces ELISA test kits and polyclonal antibodies. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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French translation
anticorps
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Gene target
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Gene symbol
IGHV3-52, IGLV5-52, SNORD115-6, SNORD115-20, SNORD115-31, SNORD115-12, SNORD115-37, SNORD115-35, SNORD115-21
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Short name
Microphthalmia Transcription Factor (MiTF), 52-56kD, Clone: C5, Mouse Monoclonal antibody- , , , Mouse, ; paraffin, IH/
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Technique
Mouse monoclonal, Antibody, paraffin, Mouse, WB, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, Western Blot on a membrane
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Species
Rat, Dogs, Horses, Humans, Mouses, Rats
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Alternative name
Microphthalmia Transcription Factor (microphthalmia-associated transcription factor), 52-56kD, clonality: complement component 5, Mouse monoclonal (antibody to-)-H. sapiens, Horse, canine, Mouse, Rat; paraffin, IH/Western Blot
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Alternative technique
antibodies, murine
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Alternative to gene target
microphthalmia-associated transcription factor, bHLHe32 and CMM8 and MI and WS2 and WS2A, MITF and IDBG-44086 and ENSG00000187098 and 4286, protein dimerization activity, nuclei, Mitf and IDBG-172461 and ENSMUSG00000035158 and 17342, BT.29882 and IDBG-644985 and ENSBTAG00000006679 and 407219
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