Gonadotropin Releasing Hormone Receptor (GnRHR), 54-60kD, Clone: A9-E4, Mouse Monoclonal antibody-Human; IF/NO flow
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Catalog numberBMDV7084
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PricePlease ask
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Size500ul
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Alternative name 1Gonadotropin Releasing Hormone Receptor (GnRHR), 54-60kD, Clone: A9-E4, Mab anti-Human; IF/NO flow
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Alternative name 2Gonadotropin Releasing Hormone Receptor (GnRHR), 54-60kD, Clone: A9-E4, Mab antibody-Human; IF/NO flow
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Alternative name 3Gonadotropin Releasing Hormone Receptor (GnRHR), 54-60kD, Clone: A9-E4, Mab antibody-Homo sapiens; IF/NO flow
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Alternative name 4anti-Gonadotropin Releasing Hormone Receptor GnRHR 54-60kD Clone: A9-E4 /NO flow
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Alternative name 5Gonadotropin Releasing Hormone Receptor GnRHR 54-60kD Clone: A9-E4 /NO flow
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Additional descriptionHormone releasing factors and releasing hormones are  signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolIGHV3-54, IGLV10-54, NFE4, PCDHGA9, SNAR-A9, RNU6-54P, UBE4B, IFIT2, UBE4A, ERGIC3
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Short nameGonadotropin Releasing Hormone Receptor (GnRHR), 54-60kD, Clone: A9-E4, Mouse Monoclonal antibody- ; IF/NO
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TechniqueMouse monoclonal, Antibody, Mouse, Flow, IF, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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SpeciesHuman, Humans, Mouses
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Alternative nameGonadotropin Releasing Hormone Receptor (GnRHR), 54-60kD, clonality: A9-E4, Mouse monoclonal (antibody to-)-H. sapiens; IF/NO flow
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Alternative techniqueantibodies, murine
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-54 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-54
- immunoglobulin heavy variable 3-54 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 10-54
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear factor, erythroid 4
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Synonyms
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Locus
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Discovery year2013-08-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotocadherin gamma subfamily A, 9
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-06-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Clustered protocadherins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall NF90 (ILF3) associated RNA A9
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Synonyms gene name
- small nuclear ILF3/NF90-associated RNA A9
- small ILF3/NF90-associated RNA A9
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-06-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small NF90 (ILF3) associated RNAs
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 54, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 54
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Locus
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Discovery year2011-08-08
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene nameubiquitination factor E4B
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Synonyms gene name
- ubiquitination factor E4B (homologous to yeast UFD2)
- ubiquitination factor E4B (UFD2 homolog, yeast)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-01-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- U-box domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterferon induced protein with tetratricopeptide repeats 2
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Synonyms gene
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Synonyms gene name
- interferon-induced protein with tetratricopeptide repeats 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-06-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tetratricopeptide repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitination factor E4A
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Synonyms gene name
- ubiquitination factor E4A (homologous to yeast UFD2)
- ubiquitination factor E4A (UFD2 homolog, yeast)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-01-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- U-box domain containing
- Armadillo like helical domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameERGIC and golgi 3
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Synonyms gene
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Synonyms gene name
- serologically defined breast cancer antigen 84
- chromosome 20 open reading frame 47
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-07-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
