Glucagon like-Peptide-1 (GLP), Clone: GLP-1/18 Mouse Monoclonal antibody-Human; DOT BLOT
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Catalog numberYIA6104.1
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PricePlease ask
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Size10 ug
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Alternative name 1Glucagon like-Peptide-1 (GLP), Clone: GLP-1/18 Mab anti-Human; DOT BLOT
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Alternative name 2Glucagon like-Peptide-1 (GLP), Clone: GLP-1/18 Mab antibody-Human; DOT BLOT
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Alternative name 3Glucagon like-Peptide-1 (GLP), Clone: GLP-1/18 Mab antibody-Homo sapiens; DOT BLOT
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Alternative name 4anti-Glucagon like- ptide-1 GLP Clone: GLP-1/18 DOT BLOT
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Alternative name 5Glucagon like- ptide-1 GLP Clone: GLP-1/18 DOT BLOT
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestWestern Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL. Dot blots are simpler than Western blot since no electrophoresis needed. It is advised to use PVDF or Nitrocellulose to deposit the protein samples that will be incubated with the blotting serum before ECL or colorimetric detection. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Additional descriptionGlucagon gene and the glucagon like peptide or GLP1 gene are peptide hormones, produced by alpha cells of the pancreas to raise the concentration of glucose in the blood as opposite of insulin, which lowers the glucose. Anti human glucagon and glp1 antibodies are used to study diabetes. Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolZGLP1, GCG, GOLGA6A, GLP1R, EHMT1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1
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Short nameGlucagon like-Peptide-1 (GLP), Clone: GLP-1/18 Mouse Monoclonal antibody- ; DOT BLOT
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TechniqueMouse monoclonal, Antibody, Dot Blot, peptide, Mouse, Blot, dot, Dot, antibodies against human proteins, antibodies for, western Blots, cicular dots, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, peptides
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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SpeciesHuman, Humans, Mouses
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Alternative nameGlucagon like-short protein sequence-1 (GLP), clonality: GLP-1/18 Mouse monoclonal (antibody to-)-H. sapiens; DOT BLOT
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Alternative techniqueantibodies, peptides, murine, western, blots
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Gene info
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Identity
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Gene
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Long gene namezinc finger GATA like protein 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2009-10-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- GATA zinc finger domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglucagon
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegolgin A6 family member A
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Synonyms gene
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Synonyms gene name
- golgi autoantigen, golgin subfamily a, member 6
- golgi autoantigen, golgin subfamily a, 6
- golgi autoantigen, golgin subfamily a, 6A
- golgin A6 family, member A
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-08-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Golgin related
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglucagon like peptide 1 receptor
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Synonyms gene name
- glucagon-like peptide 1 receptor
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Synonyms
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Locus
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Discovery year1993-03-11
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Entrez gene record
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Classification
- Glucagon receptor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameeuchromatic histone lysine methyltransferase 1
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Synonyms gene
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Synonyms gene name
- euchromatic histone methyltransferase 1
- EHMT1 intronic transcript 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-06-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SET domain containing
- Lysine methyltransferases
- Ankyrin repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
