Estrogen Receptor (ER), Inactive 8S Oligomeric state, Native Receptor in activated 4S state, ~67kD, Clone: C-542, Mouse Monoclonal antibody-Human, Mouse, Rat, Hamster; WB/IP/ICC/IHC/GS
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Catalog numberYSGSRA1010F
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PricePlease ask
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Size0.2mg
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Alternative name 1Estrogen Receptor (ER), Inactive 8S Oligomeric state, Native Receptor in activated 4S state, ~67kD, Clone: C-542, Mab anti-Human, Mouse, Rat, Hamster; WB/IP/ICC/IHC/GS
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Alternative name 2Estrogen Receptor (ER), Inactive 8S Oligomeric state, Native Receptor in activated 4S state, ~67kD, Clone: C-542, Mab antibody-Human, Mouse, Rat, Hamster; WB/IP/ICC/IHC/GS
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Alternative name 3Estrogen Receptor (ER), Inactive 8S Oligomeric state, Native Receptor in activated 4S state, ~67kD, Clone: C-542, Mab antibody-Homo sapiens, Mus musculus, Rattus norvegicus, Hamster; WB/IP/ICC/IHC/GS
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Alternative name 4anti-Estrogen Receptor ER Inactive 8S Oligomeric state Native Receptor in activated 4S state ~67kD Clone: C-542 /ICC/C/GS
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Alternative name 5Estrogen Receptor ER Inactive 8S Oligomeric state Native Receptor in activated 4S state ~67kD Clone: C-542 /ICC/C/GS
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Additional descriptionThe receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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French translationanticorps
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Gene target
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Gene symbolSMC4, MIR542, PCK1, MYB, SFTPC, VCX3B, MAF, RFX1, EGR4
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Short nameEstrogen Receptor (ER), Inactive 8S Oligomeric state, Native Receptor in activated 4S state, ~67kD, Clone: C-542, Mouse Monoclonal antibody- , Mouse, , ; /IP/ICC/IHC/GS
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TechniqueMouse monoclonal, Antibody, Mouse, ihc, IP, WB, antibodies against human proteins, antibodies for, Immunoprecipitation, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, Western Blot on a membrane
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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SpeciesHamster, Hamsters, Humans, Mouses, Rats
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Alternative nameEstrogen Receptor (ER), Inactive 8S Oligomeric state, Native Receptor in activated 4S state, ~67kD, clonality: C-542, Mouse monoclonal (antibody to-)-H. sapiens, Mouse, Rat, Hamster; Western Blot/IP/ICC/Immunohistochemistry/GS
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Alternative techniqueantibodies, murine
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Gene info
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Identity
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Gene
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Long gene namestructural maintenance of chromosomes 4
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Synonyms gene
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Synonyms gene name
- SMC4 (structural maintenance of chromosomes 4, yeast)-like 1
- SMC4 structural maintenance of chromosomes 4-like 1 (yeast)
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-02-22
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Entrez gene record
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Pubmed identfication
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Classification
- Structural maintenance of chromosomes proteins
- Condensin I subunits
- Condensin II subunits
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 542
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Synonyms gene
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Synonyms
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Locus
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Discovery year2006-02-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNAs
Gene info
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Identity
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Gene
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Long gene namephosphoenolpyruvate carboxykinase 1
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Synonyms gene name
- phosphoenolpyruvate carboxykinase 1 (soluble)
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Synonyms
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Locus
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Discovery year1990-09-10
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameMYB proto-oncogene, transcription factor
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Synonyms gene name
- v-myb avian myeloblastosis viral oncogene homolog
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Synonyms
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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Classification
- Myb/SANT domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesurfactant protein C
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Synonyms gene
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Synonyms gene name
- surfactant, pulmonary-associated protein C
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Synonyms
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Synonyms name
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Locus
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Discovery year1988-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- BRICHOS domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namevariable charge X-linked 3B
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Synonyms
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Locus
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Discovery year2005-01-11
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Classification
- Variable charge X/Y family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameMAF bZIP transcription factor
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Synonyms gene name
- v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog
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Synonyms
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Locus
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Discovery year1991-08-01
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Entrez gene record
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Pubmed identfication
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Classification
- Basic leucine zipper proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameregulatory factor X1
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Synonyms gene name
- regulatory factor X, 1 (influences HLA class II expression)
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Regulatory factor X family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameearly growth response 4
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Synonyms
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Locus
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Discovery year1992-07-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data