CD96, Mouse Monoclonal antibody-; Clone: NK92.39, flow
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Catalog number
YSRTMCA4643
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Price
Please ask
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Size
200ug
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Alternative name 1
CD96, Mab anti-; Clone: NK92.39, flow
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Alternative name 2
CD96, Mab antibody-; Clone: NK92.39, flow
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Alternative name 3
CD96, Mab antibody-; Clone: NK92.39, flow
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Alternative name 4
anti-CD96 Clone: NK92.39 flow
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Alternative name 5
CD96 Clone: NK92.39 flow
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Test
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
CD96
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Short name
CD96, Mouse Monoclonal antibody-; Clone: NK92 39,
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Technique
Mouse monoclonal, Antibody, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Species
Mouse, Mouses
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Alternative name
CD96 molecule, Mouse monoclonal (antibody to-)-; clonality: NK92.39, flow
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Alternative technique
antibodies, murine
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Alternative to gene target
CD96 molecule, TACTILE, CD96 and IDBG-49394 and ENSG00000153283 and 10225, protein binding, Plasma membranes, Cd96 and IDBG-163875 and ENSMUSG00000022657 and 84544, BT.19988 and IDBG-632160 and ENSBTAG00000014441 and 509578
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Gene info
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Identity
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Gene
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Long gene name
CD96 molecule
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
2003-01-22
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Entrez gene record
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Pubmed identfication
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Classification
- Ig-like cell adhesion molecule family
- Immunoglobulin like domain containing
- CD molecules
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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