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Alternative name 1
CD9, B-Cell (pre-), Monocyte, Platelet, p24, Clone: MM2/57, Mab anti-Human, Dog, Cat, Bovine, Horse, Rabbit, Rhesus Monkey, Swine, Biotin; flow
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Alternative name 2
CD9, B-Cell (pre-), Monocyte, Platelet, p24, Clone: MM2/57, Mab antibody-Human, Dog, Cat, Bovine, Horse, Rabbit, Rhesus Monkey, Swine, Biotin; flow
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Alternative name 3
CD9, B-Cell (pre-), Monocyte, Platelet, p24, Clone: MM2/57, Mab antibody-Homo sapiens, Canine, Cat, Bovine, Horse, Rabbit, Rhesus Monkey, Swine, Biotin; flow
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Alternative name 4
anti-CD9 B-Cell pre- Monocyte Platelet p24 Clone: MM2/57 Cat Horse Rhesus flow
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Alternative name 5
CD9 B-Cell pre- Monocyte Platelet p24 Clone: MM2/57 Cat Horse Rhesus flow
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Felix vulgaris genes or proteins used as antigen, synthetic peptide. Canine or Canis Lupus is mostly Beagle used for drug research. accurate-monoclonals produces ELISA test kits and polyclonal antibodies. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Additional description
For cells, cell lines and tissues in culture till half confluency. Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys. Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
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Test
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by accurate-monoclonals. Rabbit antibodies are very stable and can be stored for several days at room temperature. accurate-monoclonals adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Monkeys like rhesus's or the rhesus macaque (Macaca mulatta) are the most used apes in laboraty models.
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Latin name
Mus musculus, Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
CD9
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Short name
CD9, B- (pre-), Monocyte, Platelet, p24, Clone: MM2/57, Mouse Monoclonal antibody- , , Cat, , , Rabbit, , Swine, ;
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Technique
Mouse monoclonal, Antibody, Rabbit, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG. Rabbits
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Label
Biotin
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Species
Monkey, Bos Taurus genes are available form the Bovine Genome database. Cats, Dogs, Horses, Humans, Monkeys, Mouses, Rhesuses
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Alternative name
CD9 molecule, B-cellular (pre-), Monocyte, Platelet, p24, clonality: MM2/57, Mouse monoclonal (antibody to-)-H. sapiens, canine, catalase, Bovine, Horse, production species: rabbit, Rhesus Monkey, swine, biotinilated; flow
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Alternative technique
antibodies, rabbit-anti, murine
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Alternative to gene target
CD9 molecule, BTCC-1 and DRAP-27 and MIC3 and MRP-1 and TSPAN-29 and TSPAN29, CD9 and IDBG-13612 and ENSG00000010278 and 928, protein binding, Extracellular, Cd9 and IDBG-189938 and ENSMUSG00000030342 and 12527, CD9 and IDBG-640522 and ENSBTAG00000014764 and 280746
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Tissue
cell
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