CD89, Fc alpha Receptor, Clone: MIP-8a, Mouse Monoclonal antibody-Human, FITC; flow
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Catalog numberOBT0603F
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PricePlease ask
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Size0.1 mg
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Alternative name 1CD89, Fc alpha Receptor, Clone: MIP-8a, Mab anti-Human, FITC; flow
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Alternative name 2CD89, Fc alpha Receptor, Clone: MIP-8a, Mab antibody-Human, FITC; flow
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Alternative name 3CD89, Fc alpha Receptor, Clone: MIP-8a, Mab antibody-Homo sapiens, FITC; flow
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Alternative name 4anti-CD89 Fc alpha Receptor Clone: MIP-8a flow
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Alternative name 5CD89 Fc alpha Receptor Clone: MIP-8a flow
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Additional descriptionThe CD89, Fc alpha Receptor, Clone: MIP-8a, Mouse Monoclonal antibody- , FITC; is a α- or alpha protein sometimes glycoprotein present in blood. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolMIPEP, TNPO1, MIP, CCL26, TRAPPC2B, CCL15, TRAF3IP1, MAFIP, TRAPPC2, CCL23
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Short nameCD89, Fc alpha Receptor, Clone: MIP-8a, Mouse Monoclonal antibody- , FITC;
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TechniqueMouse monoclonal, Antibody, Mouse, FITC, Flow, FC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameCD89, fragment c a Receptor, clonality: MIP-8a, Mouse monoclonal (antibody to-)-H. sapiens, fluorecein; flow
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Alternative techniqueantibodies, murine, fluorescine
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Gene info
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Identity
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Gene
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Long gene namemitochondrial intermediate peptidase
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Synonyms
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Locus
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Discovery year1996-10-30
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Entrez gene record
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Pubmed identfication
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Classification
- M3 metallopeptidase family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametransportin 1
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Synonyms gene
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Synonyms gene name
- karyopherin (importin) beta 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Importins
- Armadillo like helical domain containing
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor intrinsic protein of lens fiber
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Aquaporins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 26
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 26
- chemokine (C-C motif) ligand 26
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-06-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametrafficking protein particle complex subunit 2B
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Synonyms gene
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Synonyms gene name
- spondyloepiphyseal dysplasia, late, pseudogene
- trafficking protein particle complex 2 pseudogene 1
- trafficking protein particle complex 2B
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Synonyms
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Locus
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Discovery year2000-01-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 15
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 15
- chemokine (C-C motif) ligand 15
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-02-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTRAF3 interacting protein 1
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Synonyms gene name
- TNF receptor-associated factor 3 interacting protein 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-03-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- IFT-B2 complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameMAFF interacting protein (pseudogene)
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Synonyms gene name
- MAFF interacting protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2009-02-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametrafficking protein particle complex subunit 2
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Synonyms gene
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Synonyms gene name
- spondyloepiphyseal dysplasia, late
- trafficking protein particle complex 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-01-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Trafficking protein particle complex subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 23
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 23
- chemokine (C-C motif) ligand 23
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-11-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data