CD59, Macrophage Mid-Stage Precurser Cell, Ly-C6, Clone:ER-MP 20, Rat Mouse Monoclonal antibody-Mouse, FITC
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Catalog numberBMAER-MP20F
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PricePlease ask
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Size0.1mg
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Alternative name 1CD59, Macrophage Mid-Stage Precurser Cell, Ly-C6, Clone:ER-MP 20, Rat Mab anti-Mouse, FITC
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Alternative name 2CD59, Macrophage Mid-Stage Precurser Cell, Ly-C6, Clone:ER-MP 20, Rat Mab antibody-Mouse, FITC
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Alternative name 3CD59, Macrophage Mid-Stage Precurser Cell, Ly-C6, Clone:ER-MP 20, Rattus norvegicus Mab antibody-Mus musculus, FITC
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Alternative name 4anti-CD59 Macrophage Mid-Stage Precurser Cell Ly-C6 Clone:ER-MP 20
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Alternative name 5CD59 Macrophage Mid-Stage Precurser Cell Ly-C6 Clone:ER-MP 20
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
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Additional descriptionFor cells, cell lines and tissues in culture till half confluency.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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French translationanticorps
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Gene target
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Gene symbolC6, PSMA7, EREG, MAN1B1, ESR2, ESR1, PPP1CB, CD59
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Short nameCD59, Macrophage Mid-Stage Precurser , Ly-C6, Clone:ER-MP 20, Mouse Monoclonal antibody-Mouse, FITC
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TechniqueMouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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HostRat, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesMouse, Mouses, Rats
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Alternative nameCD59 molecule, complement regulatory protein, Macrophage Mid-Stage Precurser cellular, Ly-complement component 6, clonality:ER-MP 20, Rat Mouse monoclonal (antibody to-)-Mouse, fluorecein
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Alternative techniqueantibodies, murine, fluorescine
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Alternative to gene targetcomplement component 6, C6 and IDBG-18604 and ENSG00000039537 and 729, protein binding, Extracellular, C6 and IDBG-127938 and ENSMUSG00000022181 and 12274, C6 and IDBG-629747 and ENSBTAG00000014177 and 507749
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene namecomplement C6
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Synonyms gene name
- complement component 6
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Classification
- Complement system activation components
- Sushi domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameproteasome 20S subunit alpha 7
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Synonyms gene name
- proteasome (prosome, macropain) subunit, alpha type, 7
- proteasome subunit alpha 7
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-11-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Proteasome
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameepiregulin
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-02-11
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemannosidase alpha class 1B member 1
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Synonyms gene name
- mannosidase, alpha, class 1B, member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-09-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mannosidases alpha class 1
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameestrogen receptor 2
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Synonyms gene name
- estrogen receptor 2 (ER beta)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-01-17
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Entrez gene record
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Pubmed identfication
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Classification
- Estrogen receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameestrogen receptor 1
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Estrogen receptors
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameprotein phosphatase 1 catalytic subunit beta
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Synonyms gene name
- protein phosphatase 1, catalytic subunit, beta isoform
- protein phosphatase 1, catalytic subunit, beta isozyme
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-01-22
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Entrez gene record
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Pubmed identfication
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Classification
- Protein phosphatase catalytic subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD59 molecule (CD59 blood group)
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Synonyms gene
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Synonyms gene name
- CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)
- CD59 antigen, complement regulatory protein
- CD59 molecule, complement regulatory protein
- CD59 molecule
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Synonyms
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Blood group antigens
- Complement system regulators and receptors
- CD molecules
- LY6/PLAUR domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data