CD58, LFA-3, Clone: BRIC-5, Mouse Monoclonal antibody-Human, FITC; flow/Inhibits aggregation
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Catalog numberYSRTMCA912F
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PricePlease ask
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Size0.1 mg
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Alternative name 1CD58, LFA-3, Clone: BRIC-5, Mab anti-Human, FITC; flow/Inhibits aggregation
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Alternative name 2CD58, LFA-3, Clone: BRIC-5, Mab antibody-Human, FITC; flow/Inhibits aggregation
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Alternative name 3CD58, LFA-3, Clone: BRIC-5, Mab antibody-Homo sapiens, FITC; flow/Inhibits aggregation
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Alternative name 4anti-CD58 LFA-3 Clone: BRIC-5 flow/Inhibits aggregation
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Alternative name 5CD58 LFA-3 Clone: BRIC-5 flow/Inhibits aggregation
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolCD58, TRI-AAT5-3, HS3ST5, TRY-GTA5-5, TRK-TTT3-3, ITGAL, H1-5, TRF-GAA1-5, TRP-TGG3-5, TRG-GCC2-5
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Short nameCD58, LFA-3, Clone: BRIC-5, Mouse Monoclonal antibody- , FITC; /Inhibits aggregation
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TechniqueMouse monoclonal, aggregation, Antibody, Mouse, FITC, Flow, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameCD58 molecule, LFA-3, clonality: BRIC-5, Mouse monoclonal (antibody to-)-H. sapiens, fluorecein; flow/Inhibits aggregation
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Alternative techniqueantibodies, murine, fluorescine
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Alternative to gene targetCD58 molecule, ag3 and LFA-3 and LFA3, CD58 and IDBG-101394 and ENSG00000116815 and 965, protein binding, Cell surfaces, CD58 and IDBG-634482 and ENSBTAG00000040131 and 782186
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Gene info
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Identity
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Gene
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Long gene nameCD58 molecule
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Synonyms gene
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Synonyms gene name
- CD58 antigen, (lymphocyte function-associated antigen 3)
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GenBank acession
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Locus
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Discovery year1989-04-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Ig-like cell adhesion molecule family
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Ile (anticodon AAT) 5-3
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Synonyms gene
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Synonyms gene name
- transfer RNA isoleucine 17 (anticodon AAU)
- transfer RNA-Ile (AAT) 5-5
- transfer RNA-Ile (AAT) 5-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nameheparan sulfate-glucosamine 3-sulfotransferase 5
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Synonyms gene name
- heparan sulfate (glucosamine) 3-O-sulfotransferase 5
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-06-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Sulfotransferases, membrane bound
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Tyr (anticodon GTA) 5-5
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Synonyms gene
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Synonyms gene name
- transfer RNA tyrosine 3 (anticodon GUA)
- transfer RNA-Tyr (GTA) 5-3
- transfer RNA-Tyr (GTA) 5-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Lys (anticodon TTT) 3-3
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Synonyms gene
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Synonyms gene name
- transfer RNA lysine 22 (anticodon UUU)
- transfer RNA-Lys (TTT) 3-5
- transfer RNA-Lys (TTT) 3-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nameintegrin subunit alpha L
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Synonyms gene
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Synonyms gene name
- integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
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Synonyms
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Synonyms name
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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Classification
- Integrin alpha subunits
- CD molecules
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH1.5 linker histone, cluster member
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Synonyms gene
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Synonyms gene name
- H1 histone family, member 5
- histone 1, H1b
- histone cluster 1, H1b
- histone cluster 1 H1 family member b
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-08-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H1 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Phe (anticodon GAA) 1-5
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Synonyms gene
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Synonyms gene name
- transfer RNA phenylalanine 6 (anticodon GAA)
- transfer RNA-Phe (GAA) 1-3
- transfer RNA-Phe (GAA) 1-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Pro (anticodon TGG) 3-5
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Synonyms gene
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Synonyms gene name
- transfer RNA proline 16 (anticodon UGG)
- transfer RNA-Pro (TGG) 3-4
- transfer RNA-Pro (TGG) 3-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Gly (anticodon GCC) 2-5
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Synonyms gene
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Synonyms gene name
- transfer RNA glycine 16 (anticodon GCC)
- transfer RNA-Gly (GCC) 2-3
- transfer RNA-Gly (GCC) 2-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data