-
Alternative name 1
CD55, Decay Accelarating Factor, Hematopoietic & non-hematptc Cell, Clone: BRIC216, Mab anti-Hu., Blocking; flow/IP/WB
-
Alternative name 2
CD55, Decay Accelarating Factor, Hematopoietic & non-hematptc Cell, Clone: BRIC216, Mab antibody-Hu., Blocking; flow/IP/WB
-
Alternative name 3
CD55, Decay AccelaRattus norvegicusing Factor, Hematopoietic & non-hematptc Cell, Clone: BRIC216, Mab antibody-Hu., Blocking; flow/IP/WB
-
Alternative name 4
anti-CD55 Decay Accela ing Factor Hematopoietic & non-hematptc Cell Clone: BRIC216 Hu. Blocking flow/
-
Alternative name 5
CD55 Decay Accela ing Factor Hematopoietic & non-hematptc Cell Clone: BRIC216 Hu. Blocking flow/
-
Clonality
Monoclonal Antibody
-
Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
-
Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
-
Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
-
Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Test
You can block the antibody by the specific target amino acid sequence of peptide. Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Additional description
For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Latin name
Mus musculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
CD55
-
Short name
CD55, Decay Accelarating Factor, Hematopoietic & non-hematptc , Clone: BRIC216, Mouse Monoclonal antibody-Hu., Blocking; /IP/
-
Technique
Mouse monoclonal, Antibody, Blocking, Mouse, Flow, IP, WB, antibodies against human proteins, antibodies for, blocking peptides, Immunoprecipitation, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, Western Blot on a membrane
-
Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
-
Species
Mouse, Mouses
-
Alternative name
CD55 molecule, decay accelerating factor to measure complement (Cromer blood group), Decay Accelarating Factor, Hematopoietic & non-hematptc cellular, clonality: BRIC216, Mouse monoclonal (antibody to-)-Hu., inhibiting; flow/IP/Western Blot
-
Alternative technique
control, antibodies, murine
-
Alternative to gene target
CD55 molecule, decay accelerating factor for complement (Cromer blood group), CR and CROM and DAF and TC, CD55 and IDBG-106331 and ENSG00000196352 and 1604, lipid binding, Extracellular, CD55 and IDBG-629269 and ENSBTAG00000006984 and 518609
-
Tissue
cell
-
