CD4, T-Cell, Helper/Inducer, MHC Class II Rec., HIV Rec., Clone: RPA-T4, Mouse Monoclonal antibody-Human, RPE; flow
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Catalog numberYSRTMCA1267PE
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Price:
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Sizevial
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Alternative name 1CD4, T-Cell, Helper/Inducer, MHC Class II Rec., HIV Rec., Clone: RPA-T4, Mab anti-Human, RPE; flow
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Alternative name 2CD4, T-Cell, Helper/Inducer, MHC Class II Rec., HIV Rec., Clone: RPA-T4, Mab antibody-Human, RPE; flow
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Alternative name 3CD4, T-Cell, Helper/Inducer, MHC Class II Rec., HIV Rec., Clone: RPA-T4, Mab antibody-Homo sapiens, RPE; flow
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Alternative name 4anti-CD4 T-Cell Hel r/Inducer MHC Class II Rec. HIV Rec. Clone: RPA-T4 flow
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Alternative name 5CD4 T-Cell Hel r/Inducer MHC Class II Rec. HIV Rec. Clone: RPA-T4 flow
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional descriptionCD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones. For cells, cell lines and tissues in culture till half confluency.
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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GeneThe human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome(AIDS). AIDS is a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, pre-ejaculate, or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. recombinant HIV 1 and 2 gag gene proteins p24, p17, p55 immunodominant epitopes and envelope glycoproteins, gp120 are used for production of diagnostic detection antibodies.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolRPE, CD4, B3GNT4, B4GALT4, GALNT4, B3GALT4, SERPINB3, HIVEP2
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Short nameCD4, T- , Helper/Inducer, MHC Class II Rec., HIV Rec., Clone: RPA-T4, Mouse Monoclonal antibody- , RPE;
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TechniqueMouse monoclonal, Antibody, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Labelrpe
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SpeciesHuman, Humans, Mouses
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Alternative nameCD4 molecule, T-cellular, Helper/Inducer, Major histocompabitility complex Class II Rec., human immunodeficiency virus Rec., clonality: RPA-T4, Mouse monoclonal (antibody to-)-H. sapiens, RPE; flow
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Alternative techniqueantibodies, murine
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Alternative to gene targetCD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
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Tissuecell
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Virushiv
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Gene info
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Identity
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Gene
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Long gene nameribulose-5-phosphate-3-epimerase
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD4 molecule
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Synonyms gene name
- CD4 antigen (p55)
- T-cell surface glycoprotein CD4
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
- C2-set domain containing
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameUDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-05-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Beta 3-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeta-1,4-galactosyltransferase 4
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Synonyms gene name
- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Beta 4-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepolypeptide N-acetylgalactosaminyltransferase 4
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Synonyms gene name
- UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Polypeptide N-acetylgalactosaminyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeta-1,3-galactosyltransferase 4
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Synonyms gene name
- UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4
- UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-12-01
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Entrez gene record
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Pubmed identfication
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Classification
- Beta 3-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameserpin family B member 3
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Synonyms gene
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Synonyms gene name
- squamous cell carcinoma antigen 1
- serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3
- serpin peptidase inhibitor, clade B (ovalbumin), member 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-08-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serpin peptidase inhibitors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHIVEP zinc finger 2
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Synonyms gene name
- human immunodeficiency virus type I enhancer-binding protein 2
- human immunodeficiency virus type I enhancer binding protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-10-21
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Entrez gene record
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Pubmed identfication
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Classification
- Zinc fingers C2H2-type
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data