CD4, T-Cell, Helper, Clone: W3/25, Mouse Monoclonal antibody-Rat, frozen/paraffin
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Catalog numberACL003AP
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Price:
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Size0.25mg
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Alternative name 1CD4, T-Cell, Helper, Clone: W3/25, Mab anti-Rat, frozen/paraffin
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Alternative name 2CD4, T-Cell, Helper, Clone: W3/25, Mab antibody-Rat, frozen/paraffin
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Alternative name 3CD4, T-Cell, Helper, Clone: W3/25, Mab antibody-Rattus norvegicustus norvegicus, frozen/paraffin
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Alternative name 4anti-CD4 T-Cell Hel Clone: W3/25
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Alternative name 5CD4 T-Cell Hel Clone: W3/25
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional descriptionCD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones. For cells, cell lines and tissues in culture till half confluency.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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French translationanticorps
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Gene target
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Gene symbolCD4
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Short nameCD4, T- , Helper, Clone: W3/25, Mouse Monoclonal antibody- , frozen/paraffin
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TechniqueMouse monoclonal, Antibody, paraffin, frozen, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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SpeciesRat, Mouses, Rats
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Alternative nameCD4 molecule, T-cellular, Helper, clonality: W3/25, Mouse monoclonal (antibody to-)-Rat, -25°C frozen/paraffin
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Alternative techniqueantibodies, murine
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Alternative to gene targetCD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
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Tissuecell, frozen
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Gene info
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Identity
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Gene
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Long gene nameCD4 molecule
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Synonyms gene name
- CD4 antigen (p55)
- T-cell surface glycoprotein CD4
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
- C2-set domain containing
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Thinly cut sections of frozen tissue specimens prepared with a cryostat or freezing microtome.
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Tree numbers
- E01.370.225.500.620.530.160.260
- E01.370.225.750.600.530.160.260
- E05.200.500.620.530.160.260
- E05.200.750.600.530.160.260
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data