CD3, T-Cell, Clone: UCHT-1, FITC + CD8, T-Cell, Clone: UCHT-4, PE, Mouse Monoclonal antibody-Human, dual label
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Catalog numberBYA7839-1
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PricePlease ask
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Sizevial
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Alternative name 1CD3, T-Cell, Clone: UCHT-1, FITC + CD8, T-Cell, Clone: UCHT-4, PE, Mab anti-Human, dual label
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Alternative name 2CD3, T-Cell, Clone: UCHT-1, FITC + CD8, T-Cell, Clone: UCHT-4, PE, Mab antibody-Human, dual label
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Alternative name 3CD3, T-Cell, Clone: UCHT-1, FITC + CD8, T-Cell, Clone: UCHT-4, PE, Mab antibody-Homo sapiens, dual label
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Alternative name 4anti-CD3 T-Cell Clone: UCHT-1 + CD8 T-Cell Clone: UCHT-4 dual label
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Alternative name 5CD3 T-Cell Clone: UCHT-1 + CD8 T-Cell Clone: UCHT-4 dual label
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional descriptionCytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity. For cells, cell lines and tissues in culture till half confluency. Brady printing labels are printed by a label printer for labeling with 6,5 m long cassettes.
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TestThe duality of this product lies in it's dual specificity to the gene, pH or protein. Mainly in phosphorylation by specific phosphatases. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolCD8A, IGHV7-4-1, TRV-CAC1-4, TRQ-CTG1-4, TRL-CAG1-4, TRG-GCC1-4, TRH-GTG1-4, TRE-CTC1-4, TRUND-NNN4-1, TRW-CCA4-1
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Short nameCD3, T- , Clone: UCHT-1, FITC + CD8, T- , Clone: UCHT-4, PE, Mouse Monoclonal antibody- ,
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TechniqueMouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameCD3, T-cellular, clonality: UCHT-1, fluorecein + CD8, T-cellular, clonality: UCHT-4, peroxidase, Mouse monoclonal (antibody to-)-H. sapiens, dual label
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Alternative techniqueantibodies, murine, fluorescine
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene nameCD8a molecule
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Synonyms gene
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Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 7-4-1
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Val (anticodon CAC) 1-4
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Synonyms gene
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Synonyms gene name
- transfer RNA valine 4 (anticodon CAC)
- transfer RNA-Val (CAC) 1-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-27
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Gln (anticodon CTG) 1-4
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Synonyms gene
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Synonyms gene name
- transfer RNA glutamine 24 (anticodon CUG)
- transfer RNA-Gln (CTG) 1-1
- transfer RNA-Gln (CTG) 1-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon CAG) 1-4
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 35 (anticodon CAG)
- transfer RNA-Leu (CAG) 1-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Gly (anticodon GCC) 1-4
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Synonyms gene
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Synonyms gene name
- transfer RNA glycine 15 (anticodon GCC)
- transfer RNA-Gly (GCC) 1-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-4
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 7 (anticodon GUG)
- transfer RNA-His (GTG) 1-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Glu (anticodon CTC) 1-4
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Synonyms gene
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Synonyms gene name
- transfer RNA glutamic acid 18 (anticodon CUC)
- transfer RNA-Glu (CTC) 1-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 4-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 4-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
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Classification
- Cytoplasmic transfer RNA pseudogenes
Gene info
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Identity
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Gene
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Long gene nametRNA-Trp (anticodon CCA) 4-1
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Synonyms gene
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Synonyms gene name
- transfer RNA tryptophan 5 (anticodon CCA)
- transfer RNA-Trp (CCA) 4-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
