CD3, Clone: HCD3-D2, Mouse Monoclonal antibody-Human, Biotin, mitogenic
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Catalog numberDEV103-3-3/02
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PricePlease ask
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Size200ug
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Alternative name 1CD3, Clone: HCD3-D2, Mab anti-Human, Biotin, mitogenic
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Alternative name 2CD3, Clone: HCD3-D2, Mab antibody-Human, Biotin, mitogenic
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Alternative name 3CD3, Clone: HCD3-D2, Mab antibody-Homo sapiens, Biotin, mitogenic
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Alternative name 4anti-CD3 Clone: HCD3-D2 mitogenic
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Alternative name 5CD3 Clone: HCD3-D2 mitogenic
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationBiotinylated
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolNCAPD2, IGHD2-15, SNRPD2, FANCD2, RAB3D, PDPN, MAGED2
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Short nameCD3, Clone: HCD3-D2, Mouse Monoclonal antibody- , , mitogenic
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TechniqueMouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelBiotin
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SpeciesHuman, Humans, Mouses
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Alternative nameCD3, clonality: HCD3-D2, Mouse monoclonal (antibody to-)-H. sapiens, biotinilated, mitogenic
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Alternative techniqueantibodies, murine
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Gene info
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Identity
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Gene
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Long gene namenon-SMC condensin I complex subunit D2
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Synonyms gene name
- non-SMC condensin I complex, subunit D2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-09-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Condensin I subunits
- Armadillo like helical domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy diversity 2-15
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nuclear ribonucleoprotein D2 polypeptide
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Synonyms gene
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Synonyms gene name
- small nuclear ribonucleoprotein D2 polypeptide (16.5kD)
- small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
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Synonyms
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Synonyms name
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Locus
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Discovery year1990-06-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Sm spliceosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameFA complementation group D2
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Synonyms gene
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Synonyms gene name
- Fanconi anemia complementation group D2
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-01-04
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Entrez gene record
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Pubmed identfication
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Classification
- FA complementation groups
- Armadillo like helical domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameRAB3D, member RAS oncogene family
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-06-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RAB, member RAS oncogene GTPases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepodoplanin
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-07-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameMAGE family member D2
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Synonyms gene name
- melanoma antigen family D2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-08-16
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Entrez gene record
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RefSeq identity
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Classification
- MAGE family
- Small nucleolar RNA protein coding host genes
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data