CD106, VCAM-1, INCAM-110, Endothelial Cell, Clone: DDV.1, Mouse Monoclonal antibody-Human, Biotin; flow/ELISA
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Catalog numberYSRTMCA1479B
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Price:
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Size0.5 mg
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Alternative name 1CD106, VCAM-1, INCAM-110, Endothelial Cell, Clone: DDV.1, Mab anti-Human, Biotin; flow/ELISA
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Alternative name 2CD106, VCAM-1, INCAM-110, Endothelial Cell, Clone: DDV.1, Mab antibody-Human, Biotin; flow/ELISA
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Alternative name 3CD106, VCAM-1, INCAM-110, Endothelial Cell, Clone: DDV.1, Mab antibody-Homo sapiens, Biotin; flow/ELISA
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Alternative name 4anti-CD106 VCAM-1 INCAM-110 Endothelial Cell Clone: DDV.1
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Alternative name 5CD106 VCAM-1 INCAM-110 Endothelial Cell Clone: DDV.1
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationBiotinylated
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Additional descriptionFor cells, cell lines and tissues in culture till half confluency.
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolVCAM1, AFAP1, SF3B6, DLG2, RNVU1-20, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1
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Short nameCD106, VCAM-1, INCAM-110, Endothelial , Clone: DDV 1, Mouse Monoclonal antibody- , ; /ELISA
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TechniqueMouse monoclonal, Antibody, ELISA, Mouse, Flow, antibodies against human proteins, antibodies for, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, antigen assay ELISA kit 1 Kit (96 Wells), Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelBiotin
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SpeciesHuman, Humans, Mouses
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Alternative nameCD106, VCAM-1, INCAM-110, Endothelial cellular, clonality: DDV.1, Mouse monoclonal (antibody to-)-H. sapiens, biotinilated; flow/Enzyme-linked immunosorbent assay
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Alternative techniqueantibodies, murine, elisas
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene namevascular cell adhesion molecule 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-10
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
- Ig-like cell adhesion molecule family
- Receptor ligands
- C2-set domain containing
- I-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameactin filament associated protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Pleckstrin homology domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesplicing factor 3b subunit 6
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Synonyms gene name
- splicing factor 3b, subunit 6, 14kDa
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Synonyms
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Synonyms name
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Locus
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Discovery year2014-02-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SF3b complex
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namediscs large MAGUK scaffold protein 2
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Synonyms gene name
- discs, large homolog 2, chapsyn-110 (Drosophila)
- discs, large homolog 2 (Drosophila)
- discs large homolog 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
- Membrane associated guanylate kinases
- PDZ domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, variant U1 small nuclear 20
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Synonyms
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Locus
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Discovery year2013-04-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Variant U1 small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data