AES Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)

DNA lentivector for transduction information

Lentiveral packaging plasmid DNA for non-viral plasmid transfection and direct use in plasmid expression. This DNA can alos be used for packaging into Lentiviral particles for high efficiency transduction and stably integrated expressions. GENTAUR suggests to use our ABM packaging mix LV003 of second generation virusses or the LV053, our 3rd Generation Packaging mixture. pLenti lentiviral plasmids DNAs are stored in 10milliMolar Tris/HCI with 1mM EDTA at a pH of 8 at -25 C. Vectors with selection markers like kanamycin, puromycin or cumate are available.
Lentiviral technical files
Lentivirus references
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Gene target

AES Lentiviral Vector CMV pLenti-GIII-CMV-C-term-HA
Gene symbol

MYO1G, KRT31, KRT32, KRT33B, KRT34, KRT35, ARHGAP45, KRT33A, AKAP13, PUM3
Short name

AES Lentiviral Vector ( ) (CMV) (pLenti-GIII-CMV-C-term-HA)
Technique

Vectors
Species

Human, Humans
Alternative name

amino-terminal enhancer on split Lentiviral integrating Desoxyribonucleic acid sequence (H. sapiens) (cytomegalovirus) (pLenti-GIII-cytomegalovirus-c terminus-HA)
Alternative to gene target

amino-terminal enhancer of split, AES-1 and AES-2 and ESP1 and GRG and GRG5 and TLE5, AES and IDBG-16753 and ENSG00000104964 and 166, protein binding, nuclei, Aes and IDBG-178824 and ENSMUSG00000054452 and 14797, AES and IDBG-644452 and ENSBTAG00000031363 and 505375
Virus

cmv

Identity

HGNC:13880
Gene

MYO1G
Long gene name

myosin IG
Synonyms
- HA-2
Synonyms name
- minor histocompatibility antigen HA-2
GenBank acession
- AF380932
Locus

7p13
Discovery year

2000-11-28
Entrez gene record

64005
Classification
- Myosin heavy chains, class I
- Minor histocompatibility antigens
VEGA ID

OTTHUMG00000155821

Identity

HGNC:17102
Gene

ARHGAP45
Long gene name

Rho GTPase activating protein 45
Synonyms gene
- HMHA1
Synonyms gene name
- histocompatibility (minor) HA-1
Synonyms
- KIAA0223
- HA-1
GenBank acession
- D86976
Locus

19p13.3
Discovery year

2005-12-06
Entrez gene record

23526
Pubmed identfication
- 9820596
- 9039502
Classification
- Minor histocompatibility antigens
- AH/BAR family Rho GTPase activating proteins
- F-BAR domain containing

Identity

HGNC:371
Gene

AKAP13
Long gene name

A-kinase anchoring protein 13
Synonyms gene
- LBC
Synonyms gene name
- lymphoid blast crisis oncogene
- A kinase (PRKA) anchor protein 13
Synonyms
- Ht31
- BRX
- AKAP-Lbc
- c-lbc
- PROTO-LB
- HA-3
- ARHGEF13
GenBank acession
- M90360
Locus

15q25.3
Discovery year

1999-09-16
Entrez gene record

11214
Pubmed identfication
- 9627117
- 1860836
RefSeq identity
- NM_007200
Classification
- A-kinase anchoring proteins
- Dbl family Rho GEFs
- Minor histocompatibility antigens
- Pleckstrin homology domain containing
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000172205

Identity

HGNC:29676
Gene

PUM3
Long gene name

pumilio RNA binding family member 3
Synonyms gene
- KIAA0020
Synonyms gene name
- KIAA0020
Synonyms
- XTP5
- PEN
- PUF6
- hPUF-A
- HA-8
- Puf-A
Synonyms name
- penguin homolog (Drosophila)
- minor histocompatibility antigen HA-8
GenBank acession
- AL832239
Locus

9p24.2
Discovery year

2004-01-06
Entrez gene record

9933
Pubmed identfication
- 7584026
- 7584028
- 21266351
RefSeq identity
- NM_014878
Classification
- Pumilio homology domain containing
- Minor histocompatibility antigens
VEGA ID

OTTHUMG00000019450

Name

Virus Cultivation
Concept

Scope note: Process of growing viruses in live animals, plants, or cultured cells.
Tree numbers
- E01.370.225.875.970
- E05.200.875.970
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data