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Product type
Biochemical
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Detection and sensitivity
Before using Hepes Sodium Salt, CH040 please read the package insert.
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Performance and applications
Please contact Gentaur's support by livechat, email or phone in order to receive the requested information.
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Additional information
Hepes Sodium Salt from Gentaur's specalists by email: [email protected], livechat, or phone.
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Shipping handling and storage
Hepes Sodium Salt should be kept according to label on the vial/bottle.
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.