Replication protein A 32 kDa subunit Antibody (FITC)
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Catalog number
abx106426
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Price
Please ask
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Size
100 μg
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Category
Primary Antibodies
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Clonality
Polyclonal
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Raised in
Rabbit
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Target
Replication protein A 32 kDa subunit Antibody
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Immunogen
see included datasheet or contact us
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Species reactivity
Human, Mouse, Rat (for other species please inquire)
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Purification
Affinity purified
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Applications
WB (for other applications please contact us)
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Stock availability
Shipped within 5-10 working days.
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Storage conditions
Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
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Footnote
This product is for research use only.
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Properties
If you buy Antibodies supplied by Abbexa they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Abbexa Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Abbexa FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Description
The kilo Daltons subunit weight of protein A 32 subunit Antibody (FITC) compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
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Test
Replication factor subunits and DNA replication proteins are found in DNA replication complexes like GINS, MCM,.. and replication initiators producing two identical replicas of DNA from one original DNA molecule. This process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of a double helix of two strands, and each strand of the original DNA molecule gene serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes.
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French translation
anticorps
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Gene target
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Short name
protein A 32 subunit Antibody (FITC)
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Replication protein A 32 kiloDalton functionnal sequence (antibody to-) (fluorecein)
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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