FITC-xtra

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• Catalog number
  135
• Price:

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• Size
  5 mg

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• Description
  FITC-xtra is a fluorescent labeling dye used for preparing green fluorescent bioconjugates. There are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.
• Similar product
• Shipping
  Room Temperature
• Storage
  Freezer
• Shelflife
  12 months
• Molecular Weight KD
  620.52
• Appearance
  Solid
• Solubility
  Soluble In: DMSO
• Excitation Wavelength
  496 nm
• Emission Wavelength
  524 nm
• Personal precautions
  Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
• Uses
  Laboratory chemicals *For Research Use Only*
• Technical data
• Data sheet
• Ordering
  To order FITC-xtra, please use the Cat. Nr.135 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
• Tips
  Our specialists recommend you to follow carefully the pre-registered instructions for FITC-xtra
• Properties
  This aat Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aat FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

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• Gene target
  xtra  
• Short name
  FITC-xtra
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  fluorecein-xtra
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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