FITC-xtra
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Catalog number135
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Price:
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Size5 mg
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DescriptionFITC-xtra is a fluorescent labeling dye used for preparing green fluorescent bioconjugates. There are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.
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ShippingRoom Temperature
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StorageFreezer
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Shelflife12 months
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Molecular Weight KD620.52
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AppearanceSolid
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SolubilitySoluble In: DMSO
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Excitation Wavelength496 nm
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Emission Wavelength524 nm
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Personal precautionsWear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
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UsesLaboratory chemicals *For Research Use Only*
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OrderingTo order FITC-xtra, please use the Cat. Nr.135 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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TipsOur specialists recommend you to follow carefully the pre-registered instructions for FITC-xtra
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PropertiesThis aat Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aat FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Gene target
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Short nameFITC-xtra
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TechniqueFITC, Fluorescein
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LabelFITC
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Alternative namefluorecein-xtra
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Alternative techniquefluorescine
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data