UCHL1 Antibody

• Catalog number
  6130-50
• Price
  Please ask
• Size
  50 μg

• Antibody Target
  UCHL1
• Target Alternative Name
  Neuron cytoplasmic protein 9.5, PARK5, PGP9.5, PGP 9.5, Ubiquitin carboxyl-terminal, hydrolase isozyme L1, Ubiquitin thioesterase L1, UCH-L1, Ubiquitin thioesterase L1.
• Antibody Type
  Polyclonal
• Species Reactivities
  Human
• Immunogen Sequence
  Recombinant full length protein
• Accession
  P09936
• Gene Id
  7345
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  50 µg of antibody in PBS containing 10% glycerol
• Purification
  Purified from egg yolk
• Application
  Western blot
• Positive Control
  Western Blot: Crude HEK293 cell lysate
• Application And Usages
  Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 μg/mL
• Description
  Protein ubiquitination and Deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. UCHL1, UCHL3, UCHL5/UCH37, and BRCA-1-associated protein-1 (BAP1) belong to the UCH family of DUBs, which all possess a conserved catalytic domain (UCH domain) of about 230 amino acids. Although UCHL1 and UCHL3 are the most closely related UCH family members with about 53% identity, their biochemical properties differ in that UCHL1 binds monoubiquitin and UCHL3 shows dual specificity toward both ubiquitin (Ub) and NEDD8, a Ub-like molecule. UCHL1 (PGP9.5) is a 25 kDa protein; it is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It comprises >1% of total brain protein but is almost absent from other tissues. It has been implicated both in Parkinson’s disease and in lung cancer.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  UCHL1  
• Gene symbol
  UCHL1-DT, UCHL1
• Short name
  UCHL1 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Chicken IgG
• Alternative name
  UCHL1 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:40600
• Gene
  UCHL1-DT
• Long gene name
  UCHL1 divergent transcript
• Synonyms gene
  • UCHL1-AS1
• Synonyms gene name
  • UCHL1 antisense RNA 1 (head to head)
• GenBank acession
  • DB169394
  • CN277289
• Locus
  4p13
• Discovery year
  2012-12-01
• Entrez gene record
  101410542
• RefSeq identity
  • NR_102709
• Classification
  • Divergent transcripts
• VEGA ID
  OTTHUMG00000160405

Gene info

• Identity
  HGNC:12513
• Gene
  UCHL1
• Long gene name
  ubiquitin C-terminal hydrolase L1
• Synonyms gene
  • PARK5
• Synonyms gene name
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Synonyms
  • PGP9.5
  • Uch-L1
• Synonyms name
  • ubiquitin thiolesterase
• GenBank acession
  • BC000332
• Locus
  4p13
• Discovery year
  1991-07-15
• Entrez gene record
  7345
• Pubmed identfication
  • 1840236
• RefSeq identity
  • NM_004181
• Classification
  • Ubiquitin C-terminal hydrolases
• VEGA ID
  OTTHUMG00000099377

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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