6-ROXtra fluorescence reference solution *25 uM for PCR reactions* is a purple solution which is used as a reference dye for performing PCR detections.
Molecular Weight (KD)
Soluble In: Water
Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
Laboratory chemicals *For Research Use Only*
To order 6-ROXtra fluorescence reference solution *25 uM for PCR reactions*, please use the Cat. Nr.398 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
Our specialists recommend you to follow carefully the pre-registered instructions for 6-ROXtra fluorescence reference solution *25 uM for PCR reactions*
Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
PCR, polymerase chain reaction
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
6-ROXtra reference *25 uM for PCR *
6-ROXtra fluorescence reference solution *25 uM to measure PCR test reagent reactions*
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