MCAF Antibody
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Catalog number3759-100
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PricePlease ask
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Size100 µl
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Antibody TargetMCAF
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Target Alternative NameMCAF, MBD1-containing chromatin associated factor, ATF7IP, activating transcription factor 7 interacting protein, hAM, human ATFa-associated Modulator, p621, HGNC:20092, FLJ10139, FLJ10688
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse
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Immunogen SequenceSynthetic peptide of human MCAF
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AccessionAAQ92978.1
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Gene Id55729
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AppearanceColorless liquid
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FormLiquid
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Formulation100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blotting (1:500 – 1:2000). HeLa cell nuclear extract (Cat.# 1641-1) can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the MCAF of human and mouse origins. Reactivity to other species has not been tested.
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DescriptionMBD1-Containing Chromatin-Associated Factor (MCAF) associates with methyl CpG-binding protein (MBD1) that functions to recruit methyltransferases such as SETDB1 to chromatin. MCAF is required for the transcriptional repression and heterochromatin formation mediated by MBD1 and SETDB1.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolCCL2
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Short nameMCAF Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameMCAF (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 2
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Synonyms gene
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Synonyms gene name
- small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
- chemokine (C-C motif) ligand 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1990-07-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data