L-Methionine γ-Lyase, Pseudomonas putida recombinant

• Catalog number
  7848-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  L-Methionine-α-deamino-γ-mercaptomethane-Lyase, Methioninase, METase, MGL, mdeA
• Alternative_names
  L-Methionine-α-deamino-γ-mercaptomethane-Lyase, Methioninase, METase, MGL, mdeA
• Description
  ≥90% Pure Active Recombinant L-Methionine γ-Lyase which plays a central role in sulfur amino acid metabolism
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  ≥ 0.5 U/mg.
• Biological activity
  ≥ 0.5 U/mg.
• Results
  ≥ 0.5 U/mg.
• Unit Definition
  One unit is defined as the amount of enzyme required to convert 1.0 umole of L-methionine to α-ketobutyrate, methanethiol and ammonia per minute in 20 mM potassium phosphate, pH 8.3, 150 mM NaCl at 37 °C
• Molecular Weight
  47.1 kDa (1-398 aa, NT Poly-His Tag)
• Storage Temp
  –20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from 10 mg/ml in 20 mM potassium phosphate, 150 mM NaCl, pH 8.3.
• Reconstitution Instructions
  Briefly spin down and reconstitute in water or phosphate buffer.
• Background Information
  Methionine gamma-lyase (EC 4.4.1.11) from Pseudomonas putida is a PLP-dependent enzyme which plays a central role in sulfur amino acid metabolism. METase catalyzes the α, γ-elimination of methionine to α-ketobutyrate, methanethiol, and ammonia. METase also catalyzes the α, γ-elimination of other sulfur containing amino acids, such as homocysteine, cysteine. Because of its ability to deplete methionine, METase has been considered as a viable component of cancer therapeutics against methionine-dependent tumor cells. METase has also been utilized to design drug targets for the infectious diseases caused by parasitic protozoa and anaerobic periodontal bacteria.
• Amino acid sequence
  1-398 aa, NT Poly-His Tag
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• About
  Pseudomonas is a Gram-negative bacteria. Pseudomonas genomic sequences are used in medical research. A problem in hostpital is often the Pseudomonas Aeruginosa containing multi drug resistancy properties that are transmitted through plasmids. The MDR property is transmitter by the R plasmid.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  L-Methionine   γ-Lyase   Pseudomonas   putida  
• Short name
  L-Methionine γ-Lyase, Pseudomonas putida recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Pseudomonas
• Alternative name
  L-Methionine γ-Lyase, Pseudomonas putida Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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