MOUSE Anti-MOUSE NK-1.1 ANTIGEN:Biotin Antibody

• Catalog number
  GEN214882
• Price
  Please ask
• Size
  0.025 miligrams

• Also known as
  CD161b/c
• Other names
  Killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele A; N/A; N/A; CD161 antigen-like family member B; Lymphocyte antigen 55b; Ly-55b; NKR-P1 34; Natural killer cell surface protein NKR-P1B allele SJL/BALB
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  CD161b/c
• Gene name synonims
  N/A
• Other gene names
  N/A; Klrb1b; N/A; Ly55b; Nkrp1b; Ly-55b; NKR-P1B
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgG2a
• Clone
  PK136
• Host organism
  Mouse (Mus musculus)
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  CD161b/c This item (clone PK136) recognises the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc. Although previously thought to recognise only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. This item is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. The antibody has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  Biotin (Purified IgG conjugated to Biotin - liquid)
• Concentration
  IgG concentration 0.1 miligram per 1 mililiter
• Storage and shipping
  Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
• Tested applications
  Flow Cytometry (FACS)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Antigens are peptides or recombinant or native dependent on the production method.
• Properties
  If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• French translation
  anticorps

• Gene target
  NK-1  
• Gene symbol
  B3GAT1, CD160, NCR2, NCR1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1
• Short name
  MOUSE Anti-MOUSE NK-1 1 ANTIGEN: Antibody
• Technique
  Antibody, antigen, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, antigenes, mouses
• Host
  mouse
• Label
  Biotin
• Species
  Mouse, Mouses
• Alternative name
  Anti-MOUSE ANTI MOUSE NK-1.1 ANTIGEN:Biotin
• Alternative technique
  antibodies, antigenes, murine

Gene info

• Identity
  HGNC:921
• Gene
  B3GAT1
• Long gene name
  beta-1,3-glucuronyltransferase 1
• Synonyms gene
  • CD57
  • LEU7
• Synonyms gene name
  • CD57 antigen
• Synonyms
  • GlcAT-P
  • HNK-1
  • NK-1
• Synonyms name
  • galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
  • glucuronosyltransferase P
• GenBank acession
  • AB029396
• Locus
  11q25
• Discovery year
  2000-01-07
• Entrez gene record
  27087
• RefSeq identity
  • NM_018644
• Classification
  • Beta-1,3-glucuronyltransferases
  • CD molecules
• VEGA ID
  OTTHUMG00000167180

Gene info

• Identity
  HGNC:17013
• Gene
  CD160
• Long gene name
  CD160 molecule
• Synonyms gene name
  • CD160 antigen
• Synonyms
  • BY55
  • NK1
  • NK28
• GenBank acession
  • AF060981
• Locus
  1q21.1
• Discovery year
  2003-10-02
• Entrez gene record
  11126
• Pubmed identfication
  • 9743336
  • 9973372
• RefSeq identity
  • NM_007053
• Classification
  • Immunoglobulin like domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000013749

Gene info

• Identity
  HGNC:6732
• Gene
  NCR2
• Long gene name
  natural cytotoxicity triggering receptor 2
• Synonyms gene
  • LY95
• Synonyms gene name
  • lymphocyte antigen 95 (activating NK-receptor; NK-p44)
• Synonyms
  • NK-p44
  • CD336
• GenBank acession
  • AJ225109
• Locus
  6p21.1
• Discovery year
  1999-04-09
• Entrez gene record
  9436
• Pubmed identfication
  • 10049942
• Classification
  • CD molecules
  • V-set domain containing
• VEGA ID
  OTTHUMG00000014678

Gene info

• Identity
  HGNC:6731
• Gene
  NCR1
• Long gene name
  natural cytotoxicity triggering receptor 1
• Synonyms gene
  • LY94
• Synonyms gene name
  • lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)
• Synonyms
  • NK-p46
  • NKP46
  • CD335
• GenBank acession
  • AJ001383
• Locus
  19q13.42
• Discovery year
  1999-04-09
• Entrez gene record
  9437
• Pubmed identfication
  • 9730896
• Classification
  • Immunoglobulin like domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000183212

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:31488
• Gene
  MIR101-1
• Long gene name
  microRNA 101-1
• Synonyms gene
  • MIRN101-1
• Synonyms
  • hsa-mir-101-1
• Locus
  1p31.3
• Discovery year
  2004-04-23
• Entrez gene record
  406893
• RefSeq identity
  • NR_029516
• Classification
  • MicroRNA MIR101 family

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products