Human CellExp™ VTCN1, human recombinant
-
Catalog number7412-10
-
PricePlease ask
-
Size10 ug
-
-
SynonymsB7-H4, VTCN1, B7S1, B7h.5
-
Alternative_namesB7-H4, VTCN1, B7S1, B7h.5
-
DescriptionNegatively regulates T-cell-mediated immune response and is involved in promoting epithelial cell transformation
-
RecombinantYes
-
SourceHEK293 cells
-
Purity by SDS PAGE≥90%
-
AssaySDS-PAGE
-
Biological activityMeasured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.75 - 3 µg/ml.
-
ResultsMeasured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.75 - 3 µg/ml.
-
Molecular WeightThis protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 26.1 kDa. The predicted N-terminus is Phe 29. DTT-reduced Protein migrates as 45-60 kDa due to glycosylation.
-
Storage Temp-20°C
-
ShippingGel Pack
-
Shelf Life12 months
-
AppearanceLyophilized
-
Physical form descriptionLyophilized from 0.22 um filtered solution in PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
-
Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
-
Background InformationV-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.
-
HandlingCentrifuge the vial prior to opening.
-
UsageFor Research Use Only! Not to be used in humans
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional sourceRecombinants or rec. proteins
-
Grouprecombinants
-
Gene target
-
Gene symbolVTCN1
-
Short nameCellExp™ VTCN1, recombinant
-
TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
SpeciesHumans
-
Alternative nameH. sapiens CellExp™ V-set domain containing T cellular activation inhibitor 1, H. sapiens Rec.
-
Alternative techniquerec
-
Alternative to gene targetV-set domain containing T cell activation inhibitor 1, VTCN1 and IDBG-101440 and ENSG00000134258 and 79679, protein binding, Cell surfaces, Vtcn1 and IDBG-179327 and ENSMUSG00000051076 and 242122, VTCN1 and IDBG-634392 and ENSBTAG00000014466 and 539919
-
Gene info
-
Identity
-
Gene
-
Long gene nameV-set domain containing T cell activation inhibitor 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2005-02-25
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- V-set domain containing
- B7 family
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data