Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
Purity by SDS-PAGE
Under optimal conditions, 1 mg protein L will bind approximately 5 mg human IgG. Optimal binding of Protein L to antibodies occurs at pH 7.5 over a pH range of 2.5 to 3.0.
43.2 kDa with 5 IgG binding sites
Physical form description
Lyophilized with no additives
Reconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody’s antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L can be used to detect, quantify and purify antibodies and antibody/antigen complexes. BioVision’s Recombinant Protein L contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein L detection using anti-His-tag antibodies. Protein L binds to all classes of Ig (IgG, IgM, IgA, IgE, and IgD). Protein L will also bind Single Chain Variable Fragments (ScFv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.
Centrifuge the vial prior to opening.
For Research Use Only! Not to be used in humans