E. coli DNA Ligase

Description

A NAD+ dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3′-hydroxyl and 5′-phosphoryl termini of double-stranded DNA (dsDNA).. E. coli DNA Ligase is an NAD+ dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3′-hydroxyl and 5′-phosphoryl termini of double-stranded DNA (dsDNA)
Product Highlights

1. Ligation of dsDNA with cohesive termini 2. cDNA cloning of products from second strand cDNA synthesis experiments 3. Alternative to T4 DNA Ligase when blunt-end ligation is not required 4. For ligation of blunt-end fragments use T4 DNA Ligase
Storage Temp

-20°C
Shipping

gel pack
Shelf Life

12 months
Datasheet
Notes

For research use only. Not to be used for human or animal treatment or consumption.

Gene target

DNA Ligase
Gene symbol

PRKDC, HLA-DOA
Short name

DNA Ligase
Technique

dna
Host

e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
Species

E. coli, E. coli
Alternative name

E. coli Desoxyribonucleic acid Ligase
Alternative technique

escherichia

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data