Human CellExp™ CD273 / B7-DC / PD-L2, human recombinant
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Catalog number7369-50
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PricePlease ask
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Size50 ug
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SynonymsPDL2, PD-L2, Butyrophilin B7-DC, CD273, PDCD1 ligand 2, PDCD1L2, PDCD1LG2
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Alternative_namesPDL2, PD-L2, Butyrophilin B7-DC, CD273, PDCD1 ligand 2, PDCD1L2, PDCD1LG2
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DescriptionA protein involved in the costimulatory signal essential for T-cell proliferation.
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RecombinantYes
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SourceHEK293 cells
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Biological activityMeasured by its binding ability in a functional ELISA. Immobilized rh B7-DC / PD-L2 / CD273 at 1 µg/mL (100 µl/well) can bind rhPDCD-1 with a linear range of 15 - 500 ng/ml.
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ResultsMeasured by its binding ability in a functional ELISA. Immobilized rh B7-DC / PD-L2 / CD273 at 1 µg/mL (100 µl/well) can bind rhPDCD-1 with a linear range of 15 - 500 ng/ml.
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Molecular WeightThis protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 23.4 kDa. The predicted N-terminus is Leu 20. DTT-reduced Protein migrates as 35-48 kDa due to glycosylation.
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background InformationProgrammed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolPDCD1LG2, CD274, VSIR, HHLA2, CD209, CLEC4M, RNY4P2, LRRC23, MRPL37, UBTD2
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Short nameCellExp™ CD273 / B7-DC / PD-L2, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ CD273 / B7-DC / PD-L2, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameprogrammed cell death 1 ligand 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- B7 family
- CD molecules
- Immunoglobulin like domain containing
- Receptor ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD274 molecule
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Synonyms gene
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Synonyms gene name
- programmed cell death 1 ligand 1
- CD274 antigen
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- C2-set domain containing
- V-set domain containing
- B7 family
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameV-set immunoregulatory receptor
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Synonyms gene
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Synonyms gene name
- chromosome 10 open reading frame 54
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-10-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- B7 family
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHERV-H LTR-associating 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- V-set domain containing
- B7 family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD209 molecule
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Synonyms gene name
- CD209 antigen
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-07-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Scavenger receptors
- C-type lectin domain containing
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-type lectin domain family 4 member M
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Synonyms gene
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Synonyms gene name
- CD299 antigen
- C-type lectin domain family 4, member M
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-09-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNY4 pseudogene 2
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Synonyms gene name
- RNA, Y4 small cytoplasmic (associated with Ro protein) pseudogene 2
- RNA, Ro-associated Y4 pseudogene 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-08-25
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameleucine rich repeat containing 23
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-01-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitochondrial ribosomal protein L37
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-02-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Large subunit mitochondrial ribosomal proteins
- Mitochondrial ribosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin domain containing 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-11-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data