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Gene info
Midkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines.
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Gene names
MK, ARAP, NEGF2, MK1, Midkine, Amphiregulin-associated protein, MK
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Antibodie s label
Biotin
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Purity
Anti-hMidkine specific antibody was purified by affinity chromatography and then biotinylated.
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant (human Midkine).
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Immuno application
ELISA, Western Blot
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Antibody advice
ELISA:To detect hMidkine by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 ug/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hMidkine.Sandwich:To detect hMidkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human Midkine (XP-5230) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine. Western Blot:To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Keep at
Midkine antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
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Works with
Human
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Omim nr
127116
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Ncbi nr
P21741
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Vial with antibody
Lyophilized
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NCBI GENE nr
4192
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GENE symbol
MDK
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Antigene name
midkine (neurite growth-promoting factor 2)
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Source
Homo sapiens
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Protein nr
P21741
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
