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Synonyms
MSR1, CD204, SCARA1, SR-A, phSR1, phSR2
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Alternative_names
MSR1, CD204, SCARA1, SR-A, phSR1, phSR2
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Description
Mediates the endocytosis of modified low density lipoproteins (LDLs)
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rhMSR1 at 5 µg/ml (100 µl/well) can bind biotinylated advanced glycation end products of bovine serum albumin (AGE-BSA, Cat # 2221-10) with a linear range of 1 - 150 ng/ml.
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Results
Measured by its binding ability in a functional ELISA. Immobilized rhMSR1 at 5 µg/ml (100 µl/well) can bind biotinylated advanced glycation end products of bovine serum albumin (AGE-BSA, Cat # 2221-10) with a linear range of 1 - 150 ng/ml.
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Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 42.1 kDa. The predicted N-terminus is Lys 77. DTT-reduced Protein migrates as 65-72 kDa due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
Macrophage scavenger receptor 1 (MSR1) also known as CD204, SCARA1, SR-A, and is class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. Defects in MSR1 may be a cause of prostate cancer (PC) or Barrett esophagus (BE).
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MSR1
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Short name
CellExp™ MSR1/CD204, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ macrophage scavenger receptor 1/CD204, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
macrophage scavenger receptor 1, CD204 and phSR1 and phSR2 and SCARA1 and SR-A and SRA, MSR1 and IDBG-8289 and ENSG00000038945 and 4481, low-density lipoprotein particle binding, Plasma membranes, Msr1 and IDBG-149582 and ENSMUSG00000025044 and 20288, MSR1 and IDBG-628711 and ENSBTAG00000002885 and 281311
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