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Category
Primary Antibodies
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Long description
Receptor-like protein tyrosine phosphatases (RPTPs) represent a new family of transmembrane proteins that are thought to transduce external signals by dephosphorylating phosphotyrosine residues on intracellular substRates. RPTPu is a prototypic receptor-like protein-tyrosine phosphatase (RPTP) that mediates homotypic cell-cell interactions. Intracellularly, RPTPu consists of a relatively large juxtamembrane region (about 150 amino acids) and two conserved phosphatase domains. As in most RPTPs, the first (membrane-proximal) PTP domain of RPTPu is Catalytically active, whereas its second (C-terminal) domain is inactive. RPTPu is involved in cell-cell adhesion through homophilic interactions and may also play a key role in signal transduction and growth control.
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Antibody come from
3D7 is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with ExG protein, secreted by NIH-3T3 transfected cells and purified with glutahione beads. The cells have been transfected using a fusion construct, termed ExG, encoding the entire ectodomain of the Human RPTPu molecule (aa 1-742), fused COOH terminally to GST.
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Other description
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
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Clone
3D7
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Antigen antibody binding interaction
Mouse anti Rec.Pr.Thyrosin Phosphate (RPTP) Antibody
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Antibody is raised in
Mouse (Balb/c)
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Antibody s reacts with
Human,Rat,Swine
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
Monoclonal antibodies 3G4 and 3D7 have been directed anti the extracellular domain of RPTPu.
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Research interest
Cell adhesion
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Application
Flow Cytometry,Immunofluorescence,Immunoprecipitation,Western blotting
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Antibody s suited for
3D7 is useful for western blot, immunofluorescence, immunoprecipitation and flow cytometry. Optimal antibody dilution should be determined by titration.
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Storage
Store at 4°C, or in small aliquots at -20°C.
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Relevant references
Gerben C. M. Zondag, Albert B. Reynolds§, and Wouter H. Moolenaar. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 275, No. 15, Issue of April 14, pp. 11264–11269, 2000.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
