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Target antigen
APPL
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human
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Analyses
WB,IHC-P
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Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human APPL(138-158aa NDHDAAINRYSRLSKKRENDK), identical to the related mouse and rat sequences.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the APPL Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The APPL Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
DCC-interacting protein 13-alpha(APPL1) is a protein that in humans is encoded by the APPL1 gene. The APPL1 gene is mapped to 3q21.1-p13.3. It is said to contain 709 amino acids and share 54% amino acid identity with APPL2. APPL is highly expressed in skeletal muscle, heart, ovary, and pancreas, tissues in which AKT2 mRNA is abundant. It has been regarded as an adaptor that may tether inactive AKT2 to the PI3K in the cytoplasm and thereby may expedite recruitment of AKT2 and PI3K to the cell membrane upon mitogenic stimulation.
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Related articles
1. Mitsuuchi Y, Johnson SW, Sonoda G, Tanno S, Golemis EA, Testa JR (Oct 1999). "Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2". Oncogene 18 (35): 4891–8. doi:10.1038/sj.onc.1203080. PMID 10490823. 2. Nechamen CA, Thomas RM, Dias JA (Nov 2006). "APPL1, APPL2, Akt2 and FOXO1a interact with FSHR in a potential signaling complex". Mol Cell Endocrinol 260-262: 93–9. doi:10.1016/j.mce.2006.08.014.PMC 1782224. PMID 17030088. 3. Entrez Gene: APPL1 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1".
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Gene Name
APPL1
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Protein Name
DCC-interacting protein 13-alpha(Dip13-alpha)
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Gene Full Name
adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
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Synonyms
Adapter protein containing PH domain antibody|Adaptor Protein Containing pH Domain antibody|Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody|Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody|AKT2 INTERACTOR antibody|APPL 1 antibody|APPL1 antibody|DCC interacting protein 13 alpha antibody|DCC-interacting protein 13-alpha antibody|DIP 13 alpha antibody|DIP13 alpha antibody|Dip13-alpha antibody|DIP13alpha antibody|DP13A_HUMAN antibody|KIAA1428 antibody|PTB domain and leucine zipper motif 1 antibody|PTB Domain and Leucine Zipper Motif antibody|Signaling adaptor protein DIP13alpha antibody
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Uniprot ID
Q9UKG1
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
