An antineoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. A DNA-intercalator with growth inhibitory activity against certain leukemias and sarcomas. At higher concentrations, DNA polymerase is inhibited. Inhibits cell growth and colony formation in synchronized HeLa cells. A potent inducer of apoptosis in many cell lines (1).
Actinomycin C1, Actinomycin IV, Dactinomycin
Is this a salt?
Is it cell-permeable?
>98% by TLC
DMSO (20 mg/ml)
Maximum time for storage
Protect from light and moisture
For cells, cell lines and tissues in culture till half confluency.
Actinomycin D for activation
Actinomycin D to measure cellular activation