Mouse a1M (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse a1M protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a1M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse a1M in the samples is then determined by comparing the OD of the samples to the standard curve.

• CAS Number:

  7732-18-5

• Gene Aliases:

  AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin

• Field of Research:

  Infection immunity; Immune molecule; Kidney biomarker.

• Assay Principle:

  Competitive Inhibition ELISA

• Assay Performance Time:

  2.5h

• Standard:

  200 µg/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  3.13-200 µg/mL

• Sensitivity:

  0.97 µg/mL