Caspase-8, human recombinant
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Catalog number1088-100
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Price:
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Size100units
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SynonymsCaspase-8
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Alternative_namesCaspase-8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog
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DescriptionA cysteine-aspartic acid protease involved in apoptosis
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RecombinantYes
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SourceE. coli.
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AssaySDS-PAGE
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Activity Specifications test method> 5000 units/mg
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Unit DefinitionOne unit of the recombinant caspase-8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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Storage Temp-70°C
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Shippinggel pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionLyophilized powder
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Reconstitution InstructionsReconstitute to 1 unit per µl in PBS containing 15% glycerol.
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Background InformationCaspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis. The recombinant active human caspase-8 was expressed in E. coli. The active caspase-8 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Additional descriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCASP8, CARD8, CASP8AP2
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Short nameCaspase-8, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative namecaspase-8, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namecaspase 8
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Synonyms gene name
- caspase 8, apoptosis-related cysteine protease
- caspase 8, apoptosis-related cysteine peptidase
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspases
- Death effector domain containing
- Death inducing signaling complex
- Ripoptosome
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecaspase recruitment domain family member 8
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Synonyms gene name
- caspase recruitment domain family, member 8
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspase recruitment domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecaspase 8 associated protein 2
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Synonyms gene name
- CASP8 associated protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-12-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data