anti-SMAD3 Antibody
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Catalog number49-540
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PricePlease ask
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Size0.05 mg
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Gene infoSmad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-β (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
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Gene namesSMAD3, HSPC193, HMAD-3, HsT17436, LDS1C, LDS3, Mad homolog JV15-2, MAD3, MADH3, Mothers against DPP homolog 3, JV15-2, SMA- and MAD-related protein 3, SMAD family member 3, MAD homolog 3, HSMAD3, Mad protein homolog, SMAD 3
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Antibodie s labelunlabelled serum
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PurityImmunoaffinity Chromatography
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Ab raised inRabbit
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TypePolyclonal
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Spicific topSer423/425
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AntigenSMAD3 antibody was made against amino acids 417 - 425 of SMAD3 (Human).
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Immuno applicationELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence
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Antibody adviceSMAD3 antibody can be used in Western Blot starting at 5 - 1 ug/mL, immunohistochemistry starting at 10 ug/mL, and flow cytometry.
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Suspention pH azide0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
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Keep atStore SMAD3 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.
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Works withBovine, Chicken, Xenopus, Human, Mouse, Pig, Rat, Zebrafish
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Omim nr51338669
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Ncbi nrP84022
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Vial with antibodyin solution
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NCBI GENE nr4088
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GENE symbolSMAD3
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Antigene nameSMAD family member 3
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SourceHomo sapiens
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Protein nrP84022
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSMAD3
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Short nameanti-SMAD3 Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toSMAD3 (antibody to-)
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Alternative techniqueantibodies
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Alternative to gene targetSMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125
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Gene info
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Identity
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Gene
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Long gene nameSMAD family member 3
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Synonyms gene
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Synonyms gene name
- MAD, mothers against decapentaplegic homolog 3 (Drosophila)
- SMAD, mothers against DPP homolog 3 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SMAD family
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data