GPCR GPR15 Positive Controlfor Western Blot

Vial description

purified GPCR GPR15 Positive Control
Antibody s or peptide concentration

ug/ml in stabilization buffer
Raised in

sterile environment
Reactivity

specific antigen
Immunogen

see msds
Antigen or peptide

GPCR GPR15
Original name

GPCR GPR15 Positive Control
Phosphorylation

Non phosphorylated
Epitope

specific sequence
Type

Positive Control
Antibody s relevance

WB
Antibody s application

Western Blot
Storage conditions

-20⁰C for long term storage
Genbank number

see ncbi
Protein number

P49685
Antibody s specifications
Antibody s techfiles
Test

Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
Description

Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
Group

positif

Identity

HGNC:4532
Gene

HCAR1
Long gene name

hydroxycarboxylic acid receptor 1
Synonyms gene
- GPR104
- GPR81
Synonyms gene name
- G protein-coupled receptor 104
- G protein-coupled receptor 81
Synonyms
- HCA1
- FKSG80
- TA-GPCR
- LACR1
Synonyms name
- lactate receptor 1
GenBank acession
- AF411110
Locus

12q24.31
Discovery year

2000-01-20
Entrez gene record

27198
Pubmed identfication
- 11574155
- 19047060
- 18952058
- 21454438
Classification
- Hydroxycarboxylic acid receptors

Name

Polymerase Chain Reaction
Concept

Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Tree numbers
- E05.393.620.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data