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Description
A Mouse monoclonal antibody against Human, Mouse, Rat, Rabbit, Pig, Goat, Horse Brain Derived Neurotrophic Factor (BDNF). This antibody is labeled with Cy3.
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Specifications
Host: Mouse; Species Reactivity: Human, Mouse, Rat, Rabbit, Pig, Goat, Horse; Clonality: monoclonal; Tested applications: WB; Concentration: 1mg/mL; Isotype: IgG1 Kappa; Conjugation: Cy3
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Additional_information
Sequence of the immunogen: Pro20~Arg252; Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Brain Derived Neurotrophic Factor (BDNF) Monoclonal Antibody ( , Mouse, , Rabbit, , Goat, ), can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Cloud Clone Corp antibody is well suited for fluorescent microscopy. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
cy3 conjugation kit
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Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Latin name
Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Pigs and the smaller guinea pigs are frequent used as models for humans. Rabbits are used for polyclonal antibody production by Cloud Clone Corp. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cloud Clone Corp adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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French translation
anticorps
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Gene target
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Gene symbol
BDNF, BDNF-AS
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Short name
Brain Derived Neurotrophic Factor (BDNF) Monoclonal Antibody ( , Mouse, , Rabbit, , Goat, ),
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Technique
Antibody, Rabbit, Mouse, Goat, antibodies against human proteins, antibodies for, goats, Monoclonals or monoclonal antibodies, mouses
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Host
mouse, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Label
Cy3
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Species
Horse, Goats, Horses, Humans, Mouses, Pigs and Guinea pigs, Rats
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Alternative name
Brain Derived Neurotrophic Factor (brain-derived neurotrophic factor) monoclonal (antibody to-) (H. sapiens, Mouse, Rat, production species: rabbit, swine, caprine, Horse), cyanine 3
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Alternative technique
antibodies, rabbit-anti, murine, caprine
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Alternative to gene target
brain-derived neurotrophic factor, ANON2 and BULN2, BDNF and IDBG-36817 and ENSG00000176697 and 627, growth factor activity, Extracellular, Bdnf and IDBG-195506 and ENSMUSG00000048482 and 12064, BDNF and IDBG-636102 and ENSBTAG00000008134 and 617701
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Tissue
brain
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