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Synonyms
Calpain 1
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Alternative_names
Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Micromolar-calpain
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Description
A cysteine protease involved in apoptosis activation
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Recombinant
No
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Source
Porcine Erythrocytes
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Purity by SDS PAGE
≥85%
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Assay
SDS-PAGE
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Biological activity
≥ 1000 units/mg protein prior to freezing.
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Results
≥ 1000 units/mg
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Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 minute at 25°C using the Calpain Activity Assay Kit, (K240-100).
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Molecular Weight
110 kDa
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Storage Temp
-70°C
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Shipping
gel pack
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Shelf Life
12 months
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Appearance
Liquid
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Physical form description
In 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
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Background Information
The activity of Calpain activity is attributed to two main isoforms: n-calpain and m-calpain, which are ubiquitously, expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (n-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. CAPN1 is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of the substrates involved in cytoskeletal remodeling and signal transduction. CAPN1 is activated by micromolar concentrations of calcium and inhibited by calpastatin.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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