Beclin 1 Antibody

• Catalog number
  3663-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Beclin 1
• Target Alternative Name
  BECN1 , GT197 , Beclin-1 , beclin1 , ATG6 , VPS30
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse
• Immunogen Sequence
  Synthetic peptide corresponding to residues around amino acid 25 of mouse Beclin 1
• Accession
  O88597
• Gene Id
  56208
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.2 mg/ml
• Formulation
  100 µg (0.2 mg/ml) protein A purified rabbit anti-Beclin 1 polyclonal antibody in phosphate-buffered saline (PBS) containing 1% BSA, 50% glycerol, and 0.02% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blotting (0.5-2 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes ~53 kDa beclin 1 in Jurkat cell lysate. A weak band of ~110 kDa (possibly dimer) can also be observed in Jurkat cells. In mouse intestine tissue lysate, the antibody recognizes a ~100 kDa band (possibly dimer). Reactivity to other species has not been tested.
• Description
  Beclin is a Bcl-2 binding protein. Its structure includes a Bcl-2 binding coiled-coil region, and a leucine-rich nuclear export signal (NES). Beclin protein colocalizes with intracytoplasmic organelles and nuclei in normal COS7 and MCF7 cells. Beclin interaction with Bcl-2 may be involved with host viral defense, since over expression of beclin inhibits Sindbis virus replication and expression of beclin lacking the Bcl-2 binding domain has no antiviral effects.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Beclin  
• Gene symbol
  BECN2, BECN1, BECN1P2
• Short name
  Beclin 1 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Beclin 1 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:38606
• Gene
  BECN2
• Long gene name
  beclin 2
• Synonyms gene
  • BECN1P1
• Synonyms gene name
  • beclin 1, autophagy related, pseudogene 1
• Locus
  1q43
• Discovery year
  2010-06-29
• Entrez gene record
  441925
• Pubmed identfication
  • 23954414
• RefSeq identity
  • NM_001290693
• VEGA ID
  OTTHUMG00000039863

Gene info

• Identity
  HGNC:1034
• Gene
  BECN1
• Long gene name
  beclin 1
• Synonyms gene name
  • beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
  • beclin 1, autophagy related
• Synonyms
  • ATG6
  • VPS30
• Synonyms name
  • ATG6 autophagy related 6 homolog (S. cerevisiae)
• GenBank acession
  • AF077301
• Locus
  17q21.31
• Discovery year
  1998-11-27
• Entrez gene record
  8678
• Pubmed identfication
  • 9765397
• RefSeq identity
  • NM_003766
• Classification
  • PIK3C3 complex subunits
  • MicroRNA protein coding host genes
  • Autophagy related
• VEGA ID
  OTTHUMG00000180653

Gene info

• Identity
  HGNC:51330
• Gene
  BECN1P2
• Long gene name
  beclin 1 pseudogene 2
• Synonyms gene name
  • beclin 1, autophagy related, pseudogene 2
• Locus
  6q13
• Discovery year
  2014-10-03
• Entrez gene record
  100420149
• RefSeq identity
  • NG_025950
• VEGA ID
  OTTHUMG00000014998

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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